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Literature summary extracted from

  • Pullen, K.E.; Ng, H.L.; Sung, P.Y.; Good, M.C.; Smith, S.M.; Alber, T.
    An alternate conformation and a third metal in PstP/Ppp, the M. tuberculosis PP2C-Family Ser/Thr protein phosphatase (2004), Structure, 12, 1947-1954.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 expression in Escherichia coli strain BL21(DE3) as wild-type and selenomethionine-labeled variants Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.16 purified recombinant wild-type and selenomethionine-labeled enzymes with bound phosphate, hanging drop vapour diffusion method, 18°C, 10 mg/ml protein in 50 mM NaCl, 20 mM Tris, pH 7.5, 0.5 mM Tris(2-carboxyethyl)phosphine hydrochloride, and 1 mM MnCl2, mixed with equal volume of reservoir solution containing 8% PEG 10000, 0.1 M sodium cacodylate, 0.5 mM TCEP, and 25% ethylene glycol, grown crystals are soaked in 0.1 M HEPES, pH 7.5, and 25 mM 4-nitrophenyl phosphate at 25°C, X-ray diffraction structure determination and analysis at 1.95 A resolution Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.16 Mn2+ the enzyme contains a two-metal center Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 recombinant wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by gel filtration and ion exchange chromatography Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.16 [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate catalytic mechanism, PstPpp catalytic domain structure and active site structure, structure-function relationship Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Mycobacterium tuberculosis 4-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 More the enzyme belongs to the Ser/Thr protein phosphatase PP2C-family Mycobacterium tuberculosis
3.1.3.16 PstP/Ppp
-
 Mycobacterium tuberculosis
3.1.3.16 PstPpp
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.16 25
-
 assay at Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.16 7.5
-
 assay at Mycobacterium tuberculosis