EC Number | General Stability | Organism |
---|---|---|
3.1.3.8 | 5-10% loss of activity after lyophilization | Pantoea agglomerans |
3.1.3.8 | at protein concentrations below 0.05 mg/ml a rapid loss of stability occurs | Pantoea agglomerans |
3.1.3.8 | no loss of activity after one freeze-thaw cycle | Pantoea agglomerans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.8 | Fe2+ | - |
Pantoea agglomerans | |
3.1.3.8 | Fe3+ | - |
Pantoea agglomerans | |
3.1.3.8 | myo-inositol hexakisphosphate | inhibition at substrate concentrations above 2.5 mM | Pantoea agglomerans | |
3.1.3.8 | Zn2+ | - |
Pantoea agglomerans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 0.26 | - |
D-glucose 1-phosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
3.1.3.8 | 0.34 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
3.1.3.8 | 12.4 | - |
p-nitrophenyl phosphate | 37°C, pH 4.5 | Pantoea agglomerans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.3.8 | periplasm | - |
Pantoea agglomerans | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 42000 | - |
gel filtration | Pantoea agglomerans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | D-glucose 1-phosphate + H2O | Pantoea agglomerans | strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate | D-glucose + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.8 | Pantoea agglomerans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.8 | - |
Pantoea agglomerans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 23 | - |
- |
Pantoea agglomerans |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.3.8 | -20°C, enzyme retains about 95% of initial activity after 3 months | Pantoea agglomerans |
3.1.3.8 | 4°C, protein concentration 0.1 mg/ml or greater, half-life is approximately 6 months | Pantoea agglomerans |
3.1.3.8 | room temperature, protein concentration 0.1 mg/ml or greater, half-life is 10 days | Pantoea agglomerans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.8 | D-glucose 1-phosphate + H2O | - |
Pantoea agglomerans | D-glucose + phosphate | - |
? | |
3.1.3.8 | D-glucose 1-phosphate + H2O | strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate | Pantoea agglomerans | D-glucose + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Pantoea agglomerans | ? + phosphate | - |
? | |
3.1.3.8 | p-nitrophenyl phosphate + H2O | - |
Pantoea agglomerans | p-nitrophenol + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.8 | monomer | 1 * 42000, SDS-PAGE | Pantoea agglomerans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 60 | - |
- |
Pantoea agglomerans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 22 | - |
half-life at room temperature is 10 days | Pantoea agglomerans |
3.1.3.8 | 55 | - |
90 min, no loss of activity at temperatures below | Pantoea agglomerans |
3.1.3.8 | 60 | - |
90 min, 13% loss of activity | Pantoea agglomerans |
3.1.3.8 | 65 | - |
10 min, 29% loss of activity | Pantoea agglomerans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.8 | 8.2 | - |
p-nitrophenyl phosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
3.1.3.8 | 21 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Pantoea agglomerans | |
3.1.3.8 | 101 | - |
D-glucose 1-phosphate | 37°C, pH 4.5 | Pantoea agglomerans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 4.5 | - |
- |
Pantoea agglomerans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 3 | 7 | inactive below pH 3.0 or above pH 7.0 | Pantoea agglomerans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.8 | 1.5 | - |
4°C, 24 h, 85% loss of activity | Pantoea agglomerans |
3.1.3.8 | 2.5 | 7.5 | 4°C, stable | Pantoea agglomerans |
3.1.3.8 | 4.5 | - |
4°C, 10 days, more than 95% of initial activity | Pantoea agglomerans |
3.1.3.8 | 9 | - |
4°C, 24 h, 70% loss of activity | Pantoea agglomerans |