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Literature summary extracted from

  • Greiner, R.
    Purification and properties of a phytate-degrading enzyme from Pantoea agglomerans (2004), Protein J., 23, 567-576.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.3.8 5-10% loss of activity after lyophilization Pantoea agglomerans
3.1.3.8 at protein concentrations below 0.05 mg/ml a rapid loss of stability occurs Pantoea agglomerans
3.1.3.8 no loss of activity after one freeze-thaw cycle Pantoea agglomerans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.8 Fe2+
-
Pantoea agglomerans
3.1.3.8 Fe3+
-
Pantoea agglomerans
3.1.3.8 myo-inositol hexakisphosphate inhibition at substrate concentrations above 2.5 mM Pantoea agglomerans
3.1.3.8 Zn2+
-
Pantoea agglomerans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.8 0.26
-
D-glucose 1-phosphate 37°C, pH 4.5 Pantoea agglomerans
3.1.3.8 0.34
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Pantoea agglomerans
3.1.3.8 12.4
-
p-nitrophenyl phosphate 37°C, pH 4.5 Pantoea agglomerans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 periplasm
-
Pantoea agglomerans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.8 42000
-
gel filtration Pantoea agglomerans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.8 D-glucose 1-phosphate + H2O Pantoea agglomerans strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate D-glucose + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Pantoea agglomerans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8
-
Pantoea agglomerans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.8 23
-
-
Pantoea agglomerans

Storage Stability

EC Number Storage Stability Organism
3.1.3.8 -20°C, enzyme retains about 95% of initial activity after 3 months Pantoea agglomerans
3.1.3.8 4°C, protein concentration 0.1 mg/ml or greater, half-life is approximately 6 months Pantoea agglomerans
3.1.3.8 room temperature, protein concentration 0.1 mg/ml or greater, half-life is 10 days Pantoea agglomerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 D-glucose 1-phosphate + H2O
-
Pantoea agglomerans D-glucose + phosphate
-
?
3.1.3.8 D-glucose 1-phosphate + H2O strong preference for glucose 1-phosphate over phytate, under physiological conditions glucose 1-phosphate is its most likely substrate Pantoea agglomerans D-glucose + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O
-
Pantoea agglomerans ? + phosphate
-
?
3.1.3.8 p-nitrophenyl phosphate + H2O
-
Pantoea agglomerans p-nitrophenol + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.8 monomer 1 * 42000, SDS-PAGE Pantoea agglomerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 60
-
-
Pantoea agglomerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.8 22
-
half-life at room temperature is 10 days Pantoea agglomerans
3.1.3.8 55
-
90 min, no loss of activity at temperatures below Pantoea agglomerans
3.1.3.8 60
-
90 min, 13% loss of activity Pantoea agglomerans
3.1.3.8 65
-
10 min, 29% loss of activity Pantoea agglomerans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.8 8.2
-
p-nitrophenyl phosphate 37°C, pH 4.5 Pantoea agglomerans
3.1.3.8 21
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Pantoea agglomerans
3.1.3.8 101
-
D-glucose 1-phosphate 37°C, pH 4.5 Pantoea agglomerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4.5
-
-
Pantoea agglomerans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.8 3 7 inactive below pH 3.0 or above pH 7.0 Pantoea agglomerans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.8 1.5
-
4°C, 24 h, 85% loss of activity Pantoea agglomerans
3.1.3.8 2.5 7.5 4°C, stable Pantoea agglomerans
3.1.3.8 4.5
-
4°C, 10 days, more than 95% of initial activity Pantoea agglomerans
3.1.3.8 9
-
4°C, 24 h, 70% loss of activity Pantoea agglomerans