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Literature summary extracted from

  • Kim, K.R.; Kwon, D.Y.; Yoon, S.H.; Kim, W.Y.; Kim, K.H.
    Purification, refolding, and characterization of recombinant Pseudomonas fluorescens lipase (2005), Protein Expr. Purif., 39, 124-129.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 overexpression in Escherichia coli strain BL21 in inclusion bodies Pseudomonas fluorescens

General Stability

EC Number General Stability Organism
3.1.1.3 Ca2+ is important for structural stability of the enzyme Pseudomonas fluorescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ activates the recombinant refolded enzyme, induces secondary structural changes, confers resistance to denaturation by guanidinium hydrochloride and to inactivation by DMSO and trifluoroethanol, Ca2+ is important for structural stability of the enzyme Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 additional information Pseudomonas fluorescens the enzyme is responsible for the spoilage of milk ?
-
?
3.1.1.3 additional information Pseudomonas fluorescens SIK W1 the enzyme is responsible for the spoilage of milk ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Pseudomonas fluorescens
-
-
-
3.1.1.3 Pseudomonas fluorescens SIK W1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant refolded enzyme 2.5fold from Escherichia coli strain BL21 by gel filtration and ion exchange chromatography Pseudomonas fluorescens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.1.1.3 solubilized recombinant enzyme from inclusion bodies after expression in Escherichia coli is refolded by refolding buffer incubation overnight and gel filtration at 4°C, overview Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 9264
-
purified refolded recombinant enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information the enzyme is responsible for the spoilage of milk Pseudomonas fluorescens ?
-
?
3.1.1.3 additional information the enzyme is responsible for the spoilage of milk Pseudomonas fluorescens SIK W1 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 More Ca2+ induces secondary structural changes, Ca2+ is important for structural stability of the enzyme Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
assay at Pseudomonas fluorescens