EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.3 | overexpression in Escherichia coli strain BL21 in inclusion bodies | Pseudomonas fluorescens |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.3 | Ca2+ is important for structural stability of the enzyme | Pseudomonas fluorescens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | Ca2+ | activates the recombinant refolded enzyme, induces secondary structural changes, confers resistance to denaturation by guanidinium hydrochloride and to inactivation by DMSO and trifluoroethanol, Ca2+ is important for structural stability of the enzyme | Pseudomonas fluorescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | additional information | Pseudomonas fluorescens | the enzyme is responsible for the spoilage of milk | ? | - |
? | |
3.1.1.3 | additional information | Pseudomonas fluorescens SIK W1 | the enzyme is responsible for the spoilage of milk | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Pseudomonas fluorescens | - |
- |
- |
3.1.1.3 | Pseudomonas fluorescens SIK W1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | recombinant refolded enzyme 2.5fold from Escherichia coli strain BL21 by gel filtration and ion exchange chromatography | Pseudomonas fluorescens |
EC Number | Renatured (Comment) | Organism |
---|---|---|
3.1.1.3 | solubilized recombinant enzyme from inclusion bodies after expression in Escherichia coli is refolded by refolding buffer incubation overnight and gel filtration at 4°C, overview | Pseudomonas fluorescens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 9264 | - |
purified refolded recombinant enzyme | Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | additional information | the enzyme is responsible for the spoilage of milk | Pseudomonas fluorescens | ? | - |
? | |
3.1.1.3 | additional information | the enzyme is responsible for the spoilage of milk | Pseudomonas fluorescens SIK W1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | More | Ca2+ induces secondary structural changes, Ca2+ is important for structural stability of the enzyme | Pseudomonas fluorescens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | lipase | - |
Pseudomonas fluorescens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 8 | - |
assay at | Pseudomonas fluorescens |