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Literature summary extracted from
- Expression in Trichoderma reesei and characterisation of a thermostable family 3 beta-glucosidase from the moderately thermophilic fungus Talaromyces emersonii (2004), Protein Expr. Purif., 38, 248-257.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | gene cel3a, DNA and amino acid sequence determination and analysis, subcloning and expression in Escherichia coli, expression in Trichoderma reesei | Rasamsonia emersonii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | D-glucose | competitive inhibition | Rasamsonia emersonii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.13 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Rasamsonia emersonii | |
| 3.2.1.21 | 0.18 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Aspergillus niger | |
| 3.2.1.21 | 1.1 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Trichoderma reesei | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | intracellular | - |
Rasamsonia emersonii | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 130000 | - |
2 * 130000, recombinant enzyme, SDS-PAGE | Rasamsonia emersonii |
| 3.2.1.21 | 260000 | - |
native PAGE and gel filtration, recombinant enzyme | Rasamsonia emersonii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Aspergillus niger | Q9P8F4 | - |
- |
| 3.2.1.21 | Rasamsonia emersonii | Q8TGI8 | gene cel3a | - |
| 3.2.1.21 | Rasamsonia emersonii CBS 814.70 | Q8TGI8 | gene cel3a | - |
| 3.2.1.21 | Trichoderma reesei | Q7Z9M5 | gene cel3b | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | 17 potental N-glycosylation sites | Rasamsonia emersonii |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | recombinant enzyme 24fold from Trichoderma reesei strain Rut-C30 by ultrafiltration, two steps of gel filtration, and ion exchange chromatography | Rasamsonia emersonii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 482.8 | - |
purified recombinant enzyme | Rasamsonia emersonii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rasamsonia emersonii | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rasamsonia emersonii CBS 814.70 | 4-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus niger | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Rasamsonia emersonii | 2 D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Rasamsonia emersonii CBS 814.70 | 2 D-glucose | - |
? | |
| 3.2.1.21 | cellooligosaccharides + H2O | - |
Rasamsonia emersonii | beta-D-glucose | - |
? | |
| 3.2.1.21 | cellooligosaccharides + H2O | - |
Rasamsonia emersonii CBS 814.70 | beta-D-glucose | - |
? | |
| 3.2.1.21 | additional information | substrate specificity, barley glucan and 4-nitrophenyl beta-cellobioside are poor substrates, no activity with lactose, carboxy methyl cellulose, xyloglucan, and lichenan, the enzyme is a specific beta-glucosidase and does not show beta-galactosidase activity, but it shows additional glucosyl transferase activity producing cellobiose from D-glucose and cellotetrose from cellobiose | Rasamsonia emersonii | ? | - |
? | |
| 3.2.1.21 | additional information | substrate specificity, barley glucan and 4-nitrophenyl beta-cellobioside are poor substrates, no activity with lactose, carboxy methyl cellulose, xyloglucan, and lichenan, the enzyme is a specific beta-glucosidase and does not show beta-galactosidase activity, but it shows additional glucosyl transferase activity producing cellobiose from D-glucose and cellotetrose from cellobiose | Rasamsonia emersonii CBS 814.70 | ? | - |
? | |
| 3.2.1.21 | salicin + H2O | - |
Rasamsonia emersonii | saligenin + D-glucose | - |
? | |
| 3.2.1.21 | salicin + H2O | - |
Rasamsonia emersonii CBS 814.70 | saligenin + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | 2 * 130000, recombinant enzyme, SDS-PAGE | Rasamsonia emersonii |
| 3.2.1.21 | More | secondary structure prediction and homology molecular modeling | Rasamsonia emersonii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | Cel3A | - |
Rasamsonia emersonii |
| 3.2.1.21 | Cel3b | - |
Trichoderma reesei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 70 | - |
- |
Aspergillus niger |
| 3.2.1.21 | 70 | - |
- |
Trichoderma reesei |
| 3.2.1.21 | 71.5 | - |
- |
Rasamsonia emersonii |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | 80 | - |
Rasamsonia emersonii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65 | - |
recombinant enzyme, half-life is 62 min | Rasamsonia emersonii |
| 3.2.1.21 | 75 | - |
recombinant enzyme, half-life is 18 min | Rasamsonia emersonii |
| 3.2.1.21 | 80 | - |
the recombinant enzyme retains 30% of maximal activity after 25 min | Rasamsonia emersonii |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 2226 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Rasamsonia emersonii | |
| 3.2.1.21 | 4000 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Aspergillus niger | |
| 3.2.1.21 | 8664 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 72°C, recombinant enzyme | Trichoderma reesei | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | - |
assay at | Rasamsonia emersonii |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.254 | - |
D-glucose | pH 5.0, 72°C | Rasamsonia emersonii | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Rasamsonia emersonii | isoelectric focusing, recombinant enzyme | - |
3.6 |
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