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Literature summary extracted from

  • Silva, R.N.; Asquieri, E.R.; Fernandes, K.F.
    Immobilization of Aspergillus niger glucoamylase onto a polyaniline polymer (2004), Process Biochem., 40, 1155-1159.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.3 industry immobilization of the enzyme on polyaniline polymer allows an improved catalytic performance and application of the enzyme in industrial processes Aspergillus niger

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 additional information immobilization of the enzyme on polyaniline polymer results in improved catalytic performance with decreased temperature optimum, and increased thermal stability and catalytic efficiency with increased Vmax and reduced Km, overview Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.43
-
 starch pH 4.2, 60°C, immobilized enzyme Aspergillus niger
3.2.1.3 4.76
-
 starch pH 4.2, 60°C, free enzyme Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Aspergillus niger soluble starch starch + beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Aspergillus niger
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 additional information immobilization of the enzyme on polyaniline polymer results in improved catalytic performance with decreased temperature optimum, and increased thermal stability and catalytic efficiency, overview Aspergillus niger ?
-
 ?
3.2.1.3 starch + H2O soluble starch Aspergillus niger starch + beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
 immobilized enzyme, the enzyme immobilized on polyaniline polymer shows a 10°C decreased temperature optimum Aspergillus niger
3.2.1.3 60
-
 free native enzyme Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 additional information
-
 the enzyme immobilized on polyaniline polymer shows an increased temperature stability compared to the free enzyme Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.2
-
 assay at Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 2 8 20% of maximal activity at pH 2.0 and 60% at pH 8.0, immobilized enzyme Aspergillus niger
3.2.1.3 2 7 20% of maximal activity at pH 2.0 and pH 7.0, free native enzyme Aspergillus niger