Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Parsek, M.R.; Val, D.L.; Hanzelka, B.L.; Cronan, J.E.; Greenberg, E.P.
    Acyl homoserine-lactone quorum-sensing signal generation (1999), Proc. Natl. Acad. Sci. USA, 96, 4360-4365.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.184 gene rhlI, expression in Escherichia coli strain XL 1-Blue Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.184 5'-methylthioadenosine 67% inhibition at 0.05 mM, 91% inhibition at 0.5 mM Pseudomonas aeruginosa
2.3.1.184 butyryl-S-adenosyl-L-methionine 24% inhibition at 0.05 mM, 65% inhibition at 0.5 mM Pseudomonas aeruginosa
2.3.1.184 additional information no inhibition by N-butyryl-L-homoserine-1,4-lactone, CoA, NADH, L-methionine, L-homocysteine, L-homoserine-1,4-lactone, pantothenate, L-homoserine, butyric acid, cerulenin, ATP, ADP, 4-hydroxybutyrate, butyryl-CoA, and apo-ACP Pseudomonas aeruginosa
2.3.1.184 S-Adenosyl-D-homocysteine 43% inhibition at 0.05 mM, 88% inhibition at 0.5 mM Pseudomonas aeruginosa
2.3.1.184 S-adenosyl-L-cysteine 77% inhibition at 0.05 mM, 97% inhibition at 0.5 mM Pseudomonas aeruginosa
2.3.1.184 S-adenosyl-L-homocysteine 62% inhibition at 0.05 mM, 91% inhibition at 0.5 mM Pseudomonas aeruginosa
2.3.1.184 sinefungin 58% inhibition at 0.1 mM Pseudomonas aeruginosa
2.3.1.184 [Acyl-carrier-protein] holo-[acyl-carrier-protein], 2% inhibition at 0.05 mM, 55% inhibition at 0.5 mM Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.184 additional information
-
 additional information reaction kinetics of recombinant RhlI Pseudomonas aeruginosa
2.3.1.184 0.006
-
 butyryl-[acyl-carrier protein] pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
2.3.1.184 0.008
-
 hexanoyl-[acyl-carrier protein] pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
2.3.1.184 0.014
-
 S-adenosyl-L-methionine pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
2.3.1.184 0.038
-
 butyryl-S-adenosyl-L-methionine pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
2.3.1.184 0.043
-
 octanoyl-[acyl-carrier protein] pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa
2.3.1.184 0.23
-
 butyryl-CoA pH 7.8, 37°C, recombinant RhlI Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.184 additional information Pseudomonas aeruginosa the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview ?
-
 ?
2.3.1.184 additional information Pseudomonas aeruginosa PAO-JP1 the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.184 Pseudomonas aeruginosa
-
 gene rhlI
-
2.3.1.184 Pseudomonas aeruginosa PAO-JP1
-
 gene rhlI
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.184 recombinant RhlI 95fold from Escherichia coli strain XL 1-Blue by anion and cation exchange chromatography and gel filtration Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.184 an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone RhlI shows a sequential, ordered ping-pong reaction mechanism with S-adenosyl-L-methionine binding first Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.184 0.0021
-
 purified recombinant RhlI Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.184 additional information the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview Pseudomonas aeruginosa ?
-
 ?
2.3.1.184 additional information substrate specificity of RhlI, no activity with butyrate, hexanoyl-CoA, and decanoyl-[acyl-carrier-protein], no activity with S-adenosyl-L-homocysteine, S-adenosyl-L-cysteine, L-homoserine lactone, L-homocystein, L-homoserine, and L-methionine as substrates Pseudomonas aeruginosa ?
-
 ?
2.3.1.184 additional information the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview Pseudomonas aeruginosa PAO-JP1 ?
-
 ?
2.3.1.184 additional information substrate specificity of RhlI, no activity with butyrate, hexanoyl-CoA, and decanoyl-[acyl-carrier-protein], no activity with S-adenosyl-L-homocysteine, S-adenosyl-L-cysteine, L-homoserine lactone, L-homocystein, L-homoserine, and L-methionine as substrates Pseudomonas aeruginosa PAO-JP1 ?
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-CoA
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-S-adenosyl-L-methionine
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + S-adenosyl-L-methionine
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa PAO-JP1 5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa PAO-JP1 5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?
2.3.1.184 S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein]
-
 Pseudomonas aeruginosa PAO-JP1 5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.184 acyl homoserine lactone synthase
-
 Pseudomonas aeruginosa
2.3.1.184 More the enzyme belongs to the LuxI family Pseudomonas aeruginosa
2.3.1.184 RhlI
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.184 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.184 7.8
-
 assay at Pseudomonas aeruginosa