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Literature summary extracted from
- A carbonic anhydrase from the archaeon Methanosarcina thermophila (1994), Proc. Natl. Acad. Sci. USA, 91, 6909-6913.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | expression in Escherichia coli | Methanosarcina thermophila |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | acetazolamide | IC50: 0.4 mM | Methanosarcina thermophila |  |
| 4.2.1.1 | azide | IC50: 3.4 mM | Methanosarcina thermophila | |
| 4.2.1.1 | Cyanate | IC50: 0.28 mM | Methanosarcina thermophila | |
| 4.2.1.1 | cyanide | IC50: 0.31 mM | Methanosarcina thermophila | |
| 4.2.1.1 | Iodide | - |
Methanosarcina thermophila | |
| 4.2.1.1 | sulfanilamide | - |
Methanosarcina thermophila | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | 22900 | - |
4 * 22900, calculation from nucleotide sequence | Methanosarcina thermophila |
| 4.2.1.1 | 84000 | - |
gel filtration | Methanosarcina thermophila |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | Methanosarcina thermophila | P40881 | carbonic anhydrase precursor | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | - |
Methanosarcina thermophila |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | culture condition:acetate-grown cell | - |
Methanosarcina thermophila | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | additional information | - |
- |
Methanosarcina thermophila |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.1 | tetramer | 4 * 22900, calculation from nucleotide sequence | Methanosarcina thermophila |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.1.1 | Methanosarcina thermophila | calculation from nucleotide sequence | - |
4 |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 0.28 | - |
IC50: 0.28 mM | Methanosarcina thermophila | Cyanate | |
| 4.2.1.1 | 0.31 | - |
IC50: 0.31 mM | Methanosarcina thermophila | cyanide | |
| 4.2.1.1 | 0.4 | - |
IC50: 0.4 mM | Methanosarcina thermophila | acetazolamide | |
| 4.2.1.1 | 3.4 | - |
IC50: 3.4 mM | Methanosarcina thermophila | azide | |
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Release 2023.1 (January 2023)
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