Any feedback?
Literature summary extracted from
- LeMAN4 endo-beta-mannanase from ripe tomato fruit can act as a mannan transglycosylase or hydrolase (2006), Planta, 224, 1091-1102.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.78 | NaCl | 0.3 M | Solanum lycopersicum |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | expressed in Escherichia coli | Solanum lycopersicum |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | recombinant enzyme | Solanum lycopersicum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.78 | NaCl | 1.3 M | Solanum lycopersicum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.78 | 9 | - |
Manbeta(1-4)Manbeta(1-4)Man | - |
Solanum lycopersicum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.78 | cell wall | - |
Solanum lycopersicum | 5618 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 39000 | - |
SDS-PAGE | Solanum lycopersicum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.78 | Solanum lycopersicum | - |
tomato | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.78 | salt extraction, dialysis, cation exchange chromatography | Solanum lycopersicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.78 | -20°C, MES buffer, 4 months, without loss of activity | Solanum lycopersicum |
| 3.2.1.78 | 4°C, 48h, 20% residual activity | Solanum lycopersicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.78 | azurine-crosslinked galactomannan + H2O | hydrolysis | Solanum lycopersicum | ? | - |
? | |
| 3.2.1.78 | galactoglucomannan + oligosaccharide | transglucosylation | Solanum lycopersicum | [3H]-labelled polysaccharide | - |
? | |
| 3.2.1.78 | Manbeta(1-4)Manbeta(1-4)Man + H2O | - |
Solanum lycopersicum | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.78 | endo-beta-D-mannanase | - |
Solanum lycopersicum |
| 3.2.1.78 | LeMAN4a | - |
Solanum lycopersicum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 5 | 5.3 | - |
Solanum lycopersicum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.78 | 3.5 | 6 | - |
Solanum lycopersicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
