EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.2 | Ca2+ | 50% inhibition | Thinopyrum elongatum | |
3.1.30.2 | Cu2+ | slight inhibition | Thinopyrum elongatum | |
3.1.30.2 | DTT | slight inhibition | Thinopyrum elongatum | |
3.1.30.2 | Mn2+ | slight inhibition | Thinopyrum elongatum | |
3.1.30.2 | additional information | no inhibition by NEM and iodoacetic acid | Thinopyrum elongatum | |
3.1.30.2 | NaCl | slight inhibition | Thinopyrum elongatum | |
3.1.30.2 | Ni2+ | slight inhibition | Thinopyrum elongatum | |
3.1.30.2 | putrescine | - |
Thinopyrum elongatum | |
3.1.30.2 | spermidine | - |
Thinopyrum elongatum | |
3.1.30.2 | spermine | 70% inhibition at 0.1 mM | Thinopyrum elongatum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.30.2 | additional information | no effect by Mg2+ | Thinopyrum elongatum | |
3.1.30.2 | Zn2+ | stimulates | Thinopyrum elongatum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 34000 | - |
1 * 34000, SDS-PAGE | Thinopyrum elongatum |
3.1.30.2 | 36000 | - |
gel filtration | Thinopyrum elongatum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | dsDNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids | ? | - |
? | |
3.1.30.2 | additional information | Thinopyrum elongatum | substrate specificity in descending order is ssDNA, dsDNA, RNA | ? | - |
? | |
3.1.30.2 | RNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids | ? | - |
? | |
3.1.30.2 | ssDNA + H2O | Thinopyrum elongatum | hydrolysis of nucleic acids, preference for ssDNA | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.30.2 | Thinopyrum elongatum | - |
8x = Elytrigia turcica | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.30.2 | native enzyme 265fold to homogeneity from seedlings by ammonium sulfate fractionation, anion exchange chromatography and heparin affinity chromatography | Thinopyrum elongatum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.30.2 | seedling | - |
Thinopyrum elongatum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | additional information | - |
purified enzyme | Thinopyrum elongatum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.30.2 | 5'-CCTGGCAGTT + H2O | synthetic labeled deoxydecanucleotide, cleavage of 5'-Ap*G bond and with lower activity of Gp*T, Cp*A, and Gp*G bonds | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | dsDNA + H2O | hydrolysis of nucleic acids | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | additional information | substrate specificity in descending order is ssDNA, dsDNA, RNA | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | additional information | substrate specificity, no activity with dinucleoside monophosphates, overview | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | poly(I) + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | polyA + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | polyC + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | polyG + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | polyU + H2O | synthetic homopolyribonucleotide | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | RNA + H2O | hydrolysis of nucleic acids | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | ssDNA + H2O | hydrolysis of nucleic acids, preference for ssDNA | Thinopyrum elongatum | ? | - |
? | |
3.1.30.2 | supercoiled plasmid DNA + H2O | - |
Thinopyrum elongatum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.30.2 | monomer | 1 * 34000, SDS-PAGE | Thinopyrum elongatum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.30.2 | endonuclease | - |
Thinopyrum elongatum |
3.1.30.2 | nuclease A | - |
Thinopyrum elongatum |
3.1.30.2 | plant type I nuclease | - |
Thinopyrum elongatum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 37 | - |
assay at | Thinopyrum elongatum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 60 | - |
10 min, loss of 30% of activity | Thinopyrum elongatum |
3.1.30.2 | 70 | - |
10 min, loss of 90% of activity | Thinopyrum elongatum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.30.2 | 5.5 | - |
- |
Thinopyrum elongatum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.30.2 | Thinopyrum elongatum | isoelectric focusing | - |
4.7 |