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Literature summary extracted from

  • Hellio, C.; Veron, B.; Le Gal, Y.
    Amino acid utilization by Chlamydomonas reinhardtii: Specific study of histidine (2004), Plant Physiol. Biochem., 42, 257-264.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.3.1.3 rapid decrease of activity in presence of mercaptoethanol or cysteine, 56% of initial activity after 1 d, 20% of initial activity after 2 d. In presence of DTT, the enzyme remains stable for several days Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.3 Co3+ 0.1 mM, 95% inhibition Chlamydomonas reinhardtii
4.3.1.3 Cu2+ 0.1 mM, 70% inhibition Chlamydomonas reinhardtii
4.3.1.3 cyanide 50 mM histidinol phosphate, 5 mM L-histidine and 5 mM Mn2+ protects Chlamydomonas reinhardtii
4.3.1.3 Fe2+ 0.1 mM, 60% inhibition Chlamydomonas reinhardtii
4.3.1.3 Fe3+ 0.1 mM, 60% inhibition Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.3 additional information
-
 additional information the enzyme does not exhibit classical Michaelis-Menten kinetics Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.3 Mn2+ 0.1 mM, activation to 124.5% of control Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.3 38100
-
 4 * 38100, SDS-PAGE Chlamydomonas reinhardtii
4.3.1.3 152400
-
 gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.3 Chlamydomonas reinhardtii
-
 CCAP 11/32A
-
4.3.1.3 Chlamydomonas reinhardtii CCAP 11/32A
-
 CCAP 11/32A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.3
-
 Chlamydomonas reinhardtii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.1.3 additional information grown axenically at 18°C under continous illumination. Cells grown on ammonium as the sole nitrogen source contain no histidase activity, cells grown on histidine, on histidine and glycine, or on histidine and aspartate contain histidase and exhibited similar histidase activities. When the cells are grown on histidine and ammonium, or on histidine and succinate, or on histidine and glutamate, the production of histidase is slightly delayed and reaches a lower level than when cells were grown on histidine as the sole source of nitrogen. No histidase activity was detected when C. reinhardtii was grown in the presence of acetate Chlamydomonas reinhardtii
-

Subunits

EC Number Subunits Comment Organism
4.3.1.3 tetramer 4 * 38100, SDS-PAGE Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.3 25
-
-
 Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.3 7.4 8.2
-
 Chlamydomonas reinhardtii