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Literature summary extracted from

  • Payasi, A.; Misra, P.C.; Sanwal, G.G.
    Purification and characterization of pectate lyase from banana (Musa acuminata) fruits (2006), Phytochemistry, 67, 861-869.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.2 cystine 25% activation at 1 mM, 73% activation at 5 mM Musa acuminata

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 2-mercaptoethanol 29% loss of activity at 1 mM, 67% loss of activity at 5 mM Musa acuminata
4.2.2.2 ascorbic acid 48% loss of activity at 0.1 mM, complete inactivation at 1.0 mM Musa acuminata
4.2.2.2 caffeic acid 42% inhibition at 0.01 mM, 67% inhibition at 0.05 mM Musa acuminata
4.2.2.2 catechol 24% inhibition at 0.01 mM, 52% inhibition at 0.05 mM Musa acuminata
4.2.2.2 chlorogenic acid 27% inhibition at 0.01 mM, 68% inhibition at 0.05 mM Musa acuminata
4.2.2.2 Cinnamic acid 40% inhibition at 0.01 mM, 67% inhibition at 0.05 mM Musa acuminata
4.2.2.2 dithiothreitol 27% loss of activity at 1 mM, 67% loss of activity at 5 mM Musa acuminata
4.2.2.2 ferulic acid 15% inhibition at 0.01 mM, 58% inhibition at 0.05 mM Musa acuminata
4.2.2.2 HgCl2 complete inactivation at 0.1 mM Musa acuminata
4.2.2.2 p-coumaric acid 33% inhibition at 0.01 mM, 61% inhibition at 0.05 mM Musa acuminata
4.2.2.2 PCMB 49% loss of activity at 0.01 mM, complete inactivation at 0.1 mM Musa acuminata
4.2.2.2 salicylic acid 33% inhibition at 0.01 mM, 61% inhibition at 0.05 mM Musa acuminata
4.2.2.2 Sodium metabisulfite 40% inactivation at 0.1 mM, 73% inactivation at 1 mM Musa acuminata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information KM-value for polygalacturonate is 0.4 g/l in presence of 0.7 mM Ca2+, KM-value for polygalacturonate is 0.32 g/l in presence of 0.7 mM Mg2+, KM-value for polygalacturonate is 0.45 g/l in presence of 0.7 mM Ca2+ + 0.7 mM Mg2+, KM-value for citrus pectin (7.2% methylation) is 0.77 g/l Musa acuminata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ required, can be replaced by Mn2+ or Mg2+. Optimal activity at 0.7 mM. Additive effect when any two metal ions (Mg2+, Ca2+, Mn2+) are present together Musa acuminata
4.2.2.2 Mg2+ can substitute for Ca2+ in activation. Optimal activity at 0.7 mM. Additive effect when any two metal ions (Mg2+, Ca2+, Mn2+) are present together Musa acuminata
4.2.2.2 Mn2+ can substitute for Ca2+ in activation. Optimal activity at 0.7 mM. Additive effect when any two metal ions (Mg2+, Ca2+, Mn2+) are present together Musa acuminata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 43000
-
 1 * 43000, SDS-PAGE Musa acuminata
4.2.2.2 44000
-
 gel filtration Musa acuminata

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Musa acuminata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Musa acuminata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.2 fruit ripe Musa acuminata
-

Storage Stability

EC Number Storage Stability Organism
4.2.2.2 2-5°C, pH 7.0, 0.02 M sodium phosphate buffer, stable for at least 15 days Musa acuminata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 methyl esterified pectin citrus pectin, 7% methylation Musa acuminata ?
-
 ?
4.2.2.2 polygalacturonate
-
 Musa acuminata unsaturated galacturonic acid polymer
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 monomer 1 * 43000, SDS-PAGE Musa acuminata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 37
-
-
 Musa acuminata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 37 50 37°C: optimum, 45°C: 70% of maximal activity, 50°C: 55% of maximal activity Musa acuminata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 30
-
 room temperature, activity is lost within 2 days Musa acuminata
4.2.2.2 40
-
 5 min, 5-10% loss of activity Musa acuminata
4.2.2.2 80
-
 5 min, complete inactivation Musa acuminata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8.5
-
-
 Musa acuminata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 7 10 pH 7.0: about 40% of maximal activity, pH 10.0: about 60% of maximal activity Musa acuminata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 5
-
 30% loss of activity after 3 h, 90% loss of activity after 24 h Musa acuminata
4.2.2.2 7 8.5 stable Musa acuminata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.2 Musa acuminata isoelectric focusing, pH range 3-10
-
 8