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Literature summary extracted from

  • Ohishi, K.; Yamamoto, T.; Tomofuji, T.; Tamaki, N.; Watanabe, T.
    Isolation and characterization of aminopeptidase from Capnocytophaga granulosa ATCC 51502 (2005), Oral Microbiol. Immunol., 20, 67-72.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 Ag+ complete inhibition at 0.1 mM Capnocytophaga granulosa
3.4.11.6 Cu2+ 84% inhibition at 0.1 mM Capnocytophaga granulosa
3.4.11.6 Hg2+ complete inhibition at 0.1 mM Capnocytophaga granulosa
3.4.11.6 additional information no inhibition at 0.1 mM by Mg2+ Capnocytophaga granulosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Ca2+ 26% activation at 0.1 mM Capnocytophaga granulosa
3.4.11.6 Fe3+ 29% activation at 0.1 mM Capnocytophaga granulosa
3.4.11.6 Zn2+ 34% activation at 0.1 mM Capnocytophaga granulosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 86000
-
 x * 86000, SDS-PAGE Capnocytophaga granulosa
3.4.11.6 270000
-
 gel filtration Capnocytophaga granulosa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Capnocytophaga granulosa
-
 strain ATCC 51502
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 native enzyme 159000fold by ammonium sulfate fractionation, ultrafiltration, cation exchange chromatography and gel filtration Capnocytophaga granulosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.6 297.9
-
 purified enzyme Capnocytophaga granulosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 L-Ala-2-naphthylamide + H2O
-
 Capnocytophaga granulosa L-Ala + 2-naphthylamine
-
 ?
3.4.11.6 L-Arg-2-naphthylamide + H2O
-
 Capnocytophaga granulosa L-Arg + 2-naphthylamine
-
 ?
3.4.11.6 L-Leu-2-naphthylamide + H2O
-
 Capnocytophaga granulosa L-Leu + 2-naphthylamine
-
 ?
3.4.11.6 L-Lys-2-naphthylamide + H2O
-
 Capnocytophaga granulosa L-Lys + 2-naphthylamine
-
 ?
3.4.11.6 L-Met-2-naphthylamide + H2O
-
 Capnocytophaga granulosa L-Met + 2-naphthylamine
-
 ?
3.4.11.6 additional information substrate specificity, no activity with benzoyl-Arg-2-naphthylamide, and 2-naphthylamides of Glu, Ser, and Tyr, no activity with Phe-Phe-Ala-2-naphthylamide, Leu-Gly-Gly-4-methoxy-2-naphthylamide, and Gly-Pro-Leu-2-naphthylamide, overview Capnocytophaga granulosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.6 oligomer x * 86000, SDS-PAGE Capnocytophaga granulosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.6 37
-
 assay at Capnocytophaga granulosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.6 50
-
 10 min, complete loss of activity Capnocytophaga granulosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 6.5
-
-
 Capnocytophaga granulosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.6 6.6 7 stable Capnocytophaga granulosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.6 Capnocytophaga granulosa band I, isoelectric fosusing
-
 7.5
3.4.11.6 Capnocytophaga granulosa band II, isoelectric fosusing
-
 8.8