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Literature summary extracted from

  • Sakamoto, H.; Landais, S.; Evrin, C.; Laurent-Winter, C.; Barzu, O.; Kelln, R.A.
    Structure-function relationships of UMP kinases from pyrH mutants of Gram-negative bacteria (2004), Microbiology, 150, 2153-2159.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 GTP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22
-
 Escherichia coli
2.7.4.22
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.22 A122T activity decreased to 26% of the wild-type Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 D201G activity decreased to 16% of the wild-type, significant loss of sensitivity to activation by GTP Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 D201N/E241D activity decreased to 1% of the wild-type Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 G232D resistance to heat denaturation is altered, catalytic avtivity is reduced to 17% of the wild-type Escherichia coli
2.7.4.22 R11H lowered catalytic activity, 45% of the wild-type, resistance to heat denaturation is impaired Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 UTP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Escherichia coli
-
 ADP + UDP
-
 ?
2.7.4.22 ATP + UMP Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Escherichia coli P0A7E9
-
-
2.7.4.22 Escherichia coli P0A7E9 wild type
-
2.7.4.22 Escherichia coli P0A7E9 strain KUR1244/K12 pyrH (high dose)
-
2.7.4.22 Escherichia coli P0A7E9 strain KUR1244/K12 pyrH (low dose)
-
2.7.4.22 Salmonella enterica subsp. enterica serovar Typhimurium P65933 serovar Typhimurum
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22
-
 Escherichia coli
2.7.4.22
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 0.003
-
-
 Escherichia coli
2.7.4.22 0.034
-
-
 Escherichia coli
2.7.4.22 0.1
-
-
 Escherichia coli
2.7.4.22 2.4
-
-
 Escherichia coli
2.7.4.22 7.2
-
 mutant D201G Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 50
-
 wild-type Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + UDP
-
 ?
2.7.4.22 ATP + UMP 30°C, pH 7.4 Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP 30°C, pH 7.4 Salmonella enterica subsp. enterica serovar Typhimurium ADP + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP kinase
-
 Escherichia coli
2.7.4.22 UMP kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.4.22 uridine monophosphate kinase
-
 Escherichia coli
2.7.4.22 uridine monophosphate kinase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.22 55
-
 Tm of mutant R11H Escherichia coli
2.7.4.22 57
-
 Tm of mutant G232D Escherichia coli
2.7.4.22 63
-
 Tm of the wild-type Escherichia coli
2.7.4.22 63
-
 Tm of wild-type and mutant D201G Salmonella enterica subsp. enterica serovar Typhimurium