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Literature summary extracted from

  • Park, S.Y.; Lee, S.J.; Oh, T.K.; Oh, J.W.; Koo, B.T.; Yum, D.Y.; Lee, J.K.
    AhlD, an N-acylhomoserine lactonase in Arthrobacter sp., and predicted homologues in other bacteria (2003), Microbiology, 149, 1541-1550.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 gene ahlD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha, BL21(DE3) as His-tagged enzyme, and JM109 Arthrobacter sp.
3.1.1.81 gene ahlK, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 Klebsiella pneumoniae

General Stability

EC Number General Stability Organism
3.1.1.81 the enzyme is instable during purification Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.81 30000
-
x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE Arthrobacter sp.
3.1.1.81 36000
-
x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.81 additional information Arthrobacter sp. quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
?
3.1.1.81 additional information Klebsiella pneumoniae quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
?
3.1.1.81 additional information Arthrobacter sp. IBN110 quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
?
3.1.1.81 additional information Klebsiella pneumoniae KCTC 2241 / ATCC 25306 quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Arthrobacter sp. the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 N-acyl-(S)-homoserine
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Klebsiella pneumoniae the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria N-acyl-(S)-homoserine
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Arthrobacter sp. IBN110 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 N-acyl-(S)-homoserine
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Klebsiella pneumoniae KCTC 2241 / ATCC 25306 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria N-acyl-(S)-homoserine
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Arthrobacter sp. Q7X3T2 gene ahlD
-
3.1.1.81 Arthrobacter sp. IBN110 Q7X3T2 gene ahlD
-
3.1.1.81 Klebsiella pneumoniae Q7X477 gene ahlK
-
3.1.1.81 Klebsiella pneumoniae KCTC 2241 / ATCC 25306 Q7X477 gene ahlK
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81 partially from strain IBN110 by ammonium sulfate fractionation and two steps of ion exchange chromatography, full purification failed due to enzyme instability Arthrobacter sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.81 an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine hydrolysis of the lactone ring Arthrobacter sp.
3.1.1.81 an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine hydrolysis of the lactone ring Klebsiella pneumoniae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.81 culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell
-
Arthrobacter sp.
-
3.1.1.81 culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell
-
Klebsiella pneumoniae
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.81 additional information
-
-
Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Arthrobacter sp. ?
-
?
3.1.1.81 additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Klebsiella pneumoniae ?
-
?
3.1.1.81 additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Arthrobacter sp. IBN110 ?
-
?
3.1.1.81 additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Klebsiella pneumoniae KCTC 2241 / ATCC 25306 ?
-
?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. N-3-oxododecanoyl-L-homoserine
-
?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. IBN110 N-3-oxododecanoyl-L-homoserine
-
?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. N-3-oxohexanoyl-L-homoserine
-
?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Klebsiella pneumoniae N-3-oxohexanoyl-L-homoserine
-
?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 N-3-oxohexanoyl-L-homoserine
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 Arthrobacter sp. N-acyl-(S)-homoserine
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria Klebsiella pneumoniae N-acyl-(S)-homoserine
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 Arthrobacter sp. IBN110 N-acyl-(S)-homoserine
-
?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria Klebsiella pneumoniae KCTC 2241 / ATCC 25306 N-acyl-(S)-homoserine
-
?
3.1.1.81 N-butanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. N-butanoyl-L-homoserine
-
?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. N-decanoyl-L-homoserine
-
?
3.1.1.81 N-decanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. IBN110 N-decanoyl-L-homoserine
-
?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
Klebsiella pneumoniae N-hexanoyl-L-homoserine
-
?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. N-hexanoyl-L-homoserine product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. IBN110 N-hexanoyl-L-homoserine product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 ?
3.1.1.81 N-hexanoyl-L-homoserine lactone + H2O
-
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 N-hexanoyl-L-homoserine
-
?
3.1.1.81 N-octanoyl-L-homoserine lactone + H2O
-
Arthrobacter sp. N-octanoyl-L-homoserine
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.81 ? x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE Arthrobacter sp.
3.1.1.81 More AhlD contains the conserved HXDH-H-D motif required for full enzyme activity Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 acyl homoserine degrading enzyme
-
Arthrobacter sp.
3.1.1.81 AHL-degrading enzyme
-
Arthrobacter sp.
3.1.1.81 AHL-degrading enzyme
-
Klebsiella pneumoniae
3.1.1.81 AHLase
-
Arthrobacter sp.
3.1.1.81 AHLase
-
Klebsiella pneumoniae
3.1.1.81 AhlD
-
Arthrobacter sp.
3.1.1.81 AhlK
-
Klebsiella pneumoniae
3.1.1.81 N-acylhomoserine lactonase
-
Arthrobacter sp.
3.1.1.81 N-acylhomoserine lactonase
-
Klebsiella pneumoniae