EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.81 | gene ahlD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha, BL21(DE3) as His-tagged enzyme, and JM109 | Arthrobacter sp. |
3.1.1.81 | gene ahlK, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 | Klebsiella pneumoniae |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.81 | the enzyme is instable during purification | Arthrobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | 30000 | - |
x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE | Arthrobacter sp. |
3.1.1.81 | 36000 | - |
x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE | Arthrobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.81 | additional information | Arthrobacter sp. | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
3.1.1.81 | additional information | Klebsiella pneumoniae | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
3.1.1.81 | additional information | Arthrobacter sp. IBN110 | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
3.1.1.81 | additional information | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | ? | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Arthrobacter sp. | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | N-acyl-(S)-homoserine | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Klebsiella pneumoniae | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | N-acyl-(S)-homoserine | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Arthrobacter sp. IBN110 | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | N-acyl-(S)-homoserine | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | N-acyl-(S)-homoserine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.81 | Arthrobacter sp. | Q7X3T2 | gene ahlD | - |
3.1.1.81 | Arthrobacter sp. IBN110 | Q7X3T2 | gene ahlD | - |
3.1.1.81 | Klebsiella pneumoniae | Q7X477 | gene ahlK | - |
3.1.1.81 | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | Q7X477 | gene ahlK | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.81 | partially from strain IBN110 by ammonium sulfate fractionation and two steps of ion exchange chromatography, full purification failed due to enzyme instability | Arthrobacter sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.1.81 | an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine | hydrolysis of the lactone ring | Arthrobacter sp. | |
3.1.1.81 | an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine | hydrolysis of the lactone ring | Klebsiella pneumoniae |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.81 | culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell | - |
Arthrobacter sp. | - |
3.1.1.81 | culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell | - |
Klebsiella pneumoniae | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | additional information | - |
- |
Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.81 | additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Arthrobacter sp. | ? | - |
? | |
3.1.1.81 | additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Klebsiella pneumoniae | ? | - |
? | |
3.1.1.81 | additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Arthrobacter sp. IBN110 | ? | - |
? | |
3.1.1.81 | additional information | quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | ? | - |
? | |
3.1.1.81 | N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-3-oxododecanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-3-oxododecanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. IBN110 | N-3-oxododecanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-3-oxohexanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae | N-3-oxohexanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-3-oxohexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | N-3-oxohexanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | Arthrobacter sp. | N-acyl-(S)-homoserine | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | Klebsiella pneumoniae | N-acyl-(S)-homoserine | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 | Arthrobacter sp. IBN110 | N-acyl-(S)-homoserine | - |
? | |
3.1.1.81 | N-acyl-(S)-homoserine lactone + H2O | the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria | Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | N-acyl-(S)-homoserine | - |
? | |
3.1.1.81 | N-butanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-butanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-decanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-decanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-decanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. IBN110 | N-decanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-hexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae | N-hexanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-hexanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-hexanoyl-L-homoserine | product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 | ? | |
3.1.1.81 | N-hexanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. IBN110 | N-hexanoyl-L-homoserine | product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 | ? | |
3.1.1.81 | N-hexanoyl-L-homoserine lactone + H2O | - |
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 | N-hexanoyl-L-homoserine | - |
? | |
3.1.1.81 | N-octanoyl-L-homoserine lactone + H2O | - |
Arthrobacter sp. | N-octanoyl-L-homoserine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.81 | ? | x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE | Arthrobacter sp. |
3.1.1.81 | More | AhlD contains the conserved HXDH-H-D motif required for full enzyme activity | Arthrobacter sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.81 | acyl homoserine degrading enzyme | - |
Arthrobacter sp. |
3.1.1.81 | AHL-degrading enzyme | - |
Arthrobacter sp. |
3.1.1.81 | AHL-degrading enzyme | - |
Klebsiella pneumoniae |
3.1.1.81 | AHLase | - |
Arthrobacter sp. |
3.1.1.81 | AHLase | - |
Klebsiella pneumoniae |
3.1.1.81 | AhlD | - |
Arthrobacter sp. |
3.1.1.81 | AhlK | - |
Klebsiella pneumoniae |
3.1.1.81 | N-acylhomoserine lactonase | - |
Arthrobacter sp. |
3.1.1.81 | N-acylhomoserine lactonase | - |
Klebsiella pneumoniae |