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Literature summary extracted from

  • Yu Plisova, E.; Balabanova, L.A.; Ivanova, E.P.; Kozhemyako, V.B.; Mikhailov, V.V.; Agafonova, E.V.; Rasskazov, V.A.
    A highly active alkaline phosphatase from the marine bacterium cobetia (2005), Mar. Biotechnol., 7, 173-178.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 2-mercaptoethanol 10 mM, 70% decrease in activity Cobetia marina
3.1.3.1 Cd2+ 5 mM, 30% inhibition Cobetia marina
3.1.3.1 Co2+ 5 mM, 60% inhibition Cobetia marina
3.1.3.1 Cu2+ 5 mM, 40% inhibition Cobetia marina
3.1.3.1 dithiothreitol 10 mM, 95% decrease in activity Cobetia marina
3.1.3.1 glycerol concentrations of 10%, 20% and 40% inhibit the activity by 35%, 60%, and 90% Cobetia marina
3.1.3.1 additional information iodoacetamide and NaN3 at 2-10 mM concentration have no effect on activity Cobetia marina
3.1.3.1 Ni2+ 5 mM, 27% inhibition Cobetia marina
3.1.3.1 p-chloromercuribenzoate 0.1-0.2 mM, 85%-100% inhibition Cobetia marina
3.1.3.1 Zn2+ 5 mM, complete inhibition Cobetia marina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 K+ 0.1 M, stimulates Cobetia marina
3.1.3.1 Na+ 0.1 M, stimulates Cobetia marina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 55000
-
 1 * 55000, SDS-PAGE Cobetia marina
3.1.3.1 62000
-
 gel filtration Cobetia marina

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Cobetia marina
-
-
-
3.1.3.1 Cobetia marina KMM MC-296
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1
-
 Cobetia marina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 2',3-AMP + H2O 20% of activity with p-nitrophenyl phosphate Cobetia marina ?
-
 ?
3.1.3.1 5'-AMP + H2O 90% of activity with p-nitrophenyl phosphate Cobetia marina adenosine + phosphate
-
 ?
3.1.3.1 5'-AMP + H2O 90% of activity with p-nitrophenyl phosphate Cobetia marina KMM MC-296 adenosine + phosphate
-
 ?
3.1.3.1 5'-CMP + H2O 80% of activity with p-nitrophenyl phosphate Cobetia marina cytidine + phosphate
-
 ?
3.1.3.1 5'-dCMP + H2O 80% of activity with p-nitrophenyl phosphate Cobetia marina deoxycytidine + phosphate
-
 ?
3.1.3.1 5'-GMP + H2O 40% of activity with p-nitrophenyl phosphate Cobetia marina guanosine + phosphate
-
 ?
3.1.3.1 5'-UMP + H2O 30% of activity with p-nitrophenyl phosphate Cobetia marina uridine + phosphate
-
 ?
3.1.3.1 5'-UMP + H2O 30% of activity with p-nitrophenyl phosphate Cobetia marina KMM MC-296 uridine + phosphate
-
 ?
3.1.3.1 ADP + H2O 20% of activity with p-nitrophenyl phosphate Cobetia marina ?
-
 ?
3.1.3.1 ADP + H2O 20% of activity with p-nitrophenyl phosphate Cobetia marina KMM MC-296 ?
-
 ?
3.1.3.1 alpha-glycerophosphate + H2O 60% of activity with p-nitrophenyl phosphate Cobetia marina glycerol + phosphate
-
 ?
3.1.3.1 ATP + H2O 10% of activity with p-nitrophenyl phosphate Cobetia marina ?
-
 ?
3.1.3.1 ATP + H2O 10% of activity with p-nitrophenyl phosphate Cobetia marina KMM MC-296 ?
-
 ?
3.1.3.1 beta-glycerophosphate + H2O 20% of activity with p-nitrophenyl phosphate Cobetia marina glycerol + phosphate
-
 ?
3.1.3.1 beta-glycerophosphate + H2O 20% of activity with p-nitrophenyl phosphate Cobetia marina KMM MC-296 glycerol + phosphate
-
 ?
3.1.3.1 NADP+ + H2O 20% of activity with p-nitrophenyl phosphate Cobetia marina ?
-
 ?
3.1.3.1 p-nitrophenyl phosphate + H2O
-
 Cobetia marina p-nitrophenol + phosphate
-
 ?
3.1.3.1 UDP-glucose + H2O 20% of activity with p-nitrophenyl phosphate Cobetia marina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 monomer 1 * 55000, SDS-PAGE Cobetia marina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 50
-
 stable up to, 15 min Cobetia marina
3.1.3.1 55
-
 15 min, about 20% loss of activity Cobetia marina
3.1.3.1 58
-
 15 min, about 55% loss of activity Cobetia marina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 10.3
-
-
 Cobetia marina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 9.9 10.7 about 50% of maximal activity at pH 9.9 and pH 10.7 Cobetia marina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.1 5
-
 15 min, 20% loss of activity Cobetia marina
3.1.3.1 6
-
 15 min, 10% loss of activity Cobetia marina
3.1.3.1 7 11 15 min, stable Cobetia marina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.1 Cobetia marina chromatofocusing
-
 4.5