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Literature summary extracted from

  • Zareen, N.; Yan, H.; Hopkinson, A.; Levinger, L.
    Residues in the conserved His domain of fruit fly tRNase Z that function in catalysis are not involved in substrate recognition or binding (2005), J. Mol. Biol., 350, 189-199.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.11 expression in insect SF9 cells Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.11 A493T processing efficiency similar to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 A501T processing efficiency similar to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 D502T processing efficiency reduced 7420fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 G506A processing efficiency reduced 11fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 H498A processing efficiency reduced 1950fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 H500A processing efficiency reduced 3379fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 H503A processing efficiency reduced 817fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 H504A processing efficiency reduced 7fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 I494A processing efficiency similar to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 I505A processing efficiency similar to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 I508A processing efficiency reduced 6fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 L491A processing efficiency reduced 7fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 L499A processing efficiency similar to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 L507A processing efficiency reduced 6fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 Q492A processing efficiency similar to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 S497A processing efficiency reduced 495fold compared to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 V496A processing efficiency similar to wild-type tRNase Z Drosophila melanogaster
3.1.26.11 Y495A processing efficiency similar to wild-type tRNase Z Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.11 0.000155
-
 pre-tRNAHis 25 mM Tris-HCl pH 7.0, 1 mM MgCl2, 1 mM DTT, 50 mM KCl at 28°C Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.11 82920
-
 calculated from sequence Drosophila melanogaster
3.1.26.11 90000
-
 SDS-PAGE Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.11 Drosophila melanogaster Q8MKW7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.11 His-tag fusion, Ni-affinity chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.11 cell culture
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.11 pre-tRNAHis + H2O
-
 Drosophila melanogaster ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.11 monomer 1 * 90000, SDS-PAGE Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
3.1.26.11 tRNaseZ
-
 Drosophila melanogaster