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Literature summary extracted from

  • Kreusch, A.; Spraggon, G.; Lee, C.C.; Klock, H.; McMullan, D.; Ng, K.; Shin, T.; Vincent, J.; Warner, I.; Ericson, C.; Lesley, S.A.
    Structure analysis of peptide deformylases from Streptococcus pneumoniae, Staphylococcus aureus, Thermotoga maritima and Pseudomonas aeruginosa: snapshots of the oxygen sensitivity of peptide deformylase (2003), J. Mol. Biol., 330, 309-321.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.88 drug development potential target or the development of new antibacterial agents Plasmodium falciparum
3.5.1.88 drug development potential target or the development of new antibacterial agents Escherichia coli
3.5.1.88 drug development potential target or the development of new antibacterial agents Pseudomonas aeruginosa
3.5.1.88 drug development potential target or the development of new antibacterial agents Staphylococcus aureus
3.5.1.88 drug development potential target or the development of new antibacterial agents Streptococcus pneumoniae
3.5.1.88 drug development potential target or the development of new antibacterial agents Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.88 expressed as His-tag fusion protein in Escherichia coli Pseudomonas aeruginosa
3.5.1.88 expressed as His-tag fusion protein in Escherichia coli Staphylococcus aureus
3.5.1.88 expressed as His-tag fusion protein in Escherichia coli Streptococcus pneumoniae
3.5.1.88 expressed as His-tag fusion protein in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.88 sitting drop vapor diffusion method Plasmodium falciparum
3.5.1.88 sitting drop vapor diffusion method Escherichia coli
3.5.1.88 sitting drop vapor diffusion method Pseudomonas aeruginosa
3.5.1.88 sitting drop vapor diffusion method Staphylococcus aureus
3.5.1.88 sitting drop vapor diffusion method Streptococcus pneumoniae
3.5.1.88 sitting drop vapor diffusion method Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.88 Co2+ can replace Fe2+ without loss of activity, enhances stability Plasmodium falciparum
3.5.1.88 Co2+ can replace Fe2+ without loss of activity, enhances stability Escherichia coli
3.5.1.88 Co2+ can replace Fe2+ without loss of activity, enhances stability Staphylococcus aureus
3.5.1.88 Co2+ can replace Fe2+ without loss of activity, enhances stability Streptococcus pneumoniae
3.5.1.88 Co2+ can replace Fe2+ without loss of activity, enhances stability Thermotoga maritima
3.5.1.88 Fe2+ required, irreversible oxidation to Fe3+ results in almost complete loss of activity Plasmodium falciparum
3.5.1.88 Fe2+ required, irreversible oxidation to Fe3+ results in almost complete loss of activity Escherichia coli
3.5.1.88 Fe2+ required, irreversible oxidation to Fe3+ results in almost complete loss of activity Staphylococcus aureus
3.5.1.88 Fe2+ required, irreversible oxidation to Fe3+ results in almost complete loss of activity Streptococcus pneumoniae
3.5.1.88 Fe2+ required, irreversible oxidation to Fe3+ results in almost complete loss of activity Thermotoga maritima
3.5.1.88 Ni2+ can replace Zn2+ Pseudomonas aeruginosa
3.5.1.88 Ni2+ can replace Fe2+ without loss of activity, enhances stability Plasmodium falciparum
3.5.1.88 Ni2+ can replace Fe2+ without loss of activity, enhances stability Escherichia coli
3.5.1.88 Ni2+ can replace Fe2+ without loss of activity, enhances stability Staphylococcus aureus
3.5.1.88 Ni2+ can replace Fe2+ without loss of activity, enhances stability Streptococcus pneumoniae
3.5.1.88 Ni2+ can replace Fe2+ without loss of activity, enhances stability Thermotoga maritima
3.5.1.88 Zn2+ required Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Plasmodium falciparum involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Escherichia coli involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Pseudomonas aeruginosa involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Staphylococcus aureus involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Streptococcus pneumoniae involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Thermotoga maritima involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Staphylococcus aureus ATCC 2913 involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides formate + L-methionine-polypeptide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.88 Escherichia coli P0A6K3
-
-
3.5.1.88 Plasmodium falciparum
-
-
-
3.5.1.88 Pseudomonas aeruginosa Q9I7A8 strain ATCC27853
-
3.5.1.88 Staphylococcus aureus P68826 strain ATCC2913
-
3.5.1.88 Staphylococcus aureus ATCC 2913 P68826 strain ATCC2913
-
3.5.1.88 Streptococcus pneumoniae Q9F2F0 strain ATCC6305
-
3.5.1.88 Thermotoga maritima P96113
-
-
3.5.1.88 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 P96113
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.5.1.88 instable towards oxidation due to the oxidation of the metal ligating cysteine residue Plasmodium falciparum
3.5.1.88 instable towards oxidation due to the oxidation of the metal ligating cysteine residue Escherichia coli
3.5.1.88 instable towards oxidation due to the oxidation of the metal ligating cysteine residue Pseudomonas aeruginosa
3.5.1.88 instable towards oxidation due to the oxidation of the metal ligating cysteine residue Staphylococcus aureus
3.5.1.88 instable towards oxidation due to the oxidation of the metal ligating cysteine residue Streptococcus pneumoniae
3.5.1.88 instable towards oxidation due to the oxidation of the metal ligating cysteine residue Thermotoga maritima

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.88 recombinant enzyme using His-tag Pseudomonas aeruginosa
3.5.1.88 recombinant enzyme using His-tag Staphylococcus aureus
3.5.1.88 recombinant enzyme using His-tag Streptococcus pneumoniae
3.5.1.88 recombinant enzyme using His-tag Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Plasmodium falciparum formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Escherichia coli formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Pseudomonas aeruginosa formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Staphylococcus aureus formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Streptococcus pneumoniae formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Thermotoga maritima formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Staphylococcus aureus ATCC 2913 formate + L-methionine-polypeptide
-
 ?
3.5.1.88 N-formyl-L-methionine-polypeptide + H2O involved in polypeptide synthesis by removal of the formyl-group from methionine in growing polypeptides Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 formate + L-methionine-polypeptide
-
 ?