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Literature summary extracted from

  • Park, H.; Kim, N.; Han, M.J.; Bae, E.; Kim, D.
    Purification and characterization of two novel beta-D-glucuronidases converting glycyrrhizin to 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide from Streptococcus LJ-22 (2005), J. Microbiol. Biotechnol., 15, 792-799.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 4-chloromercuriphenylsulfonic acid inhibition of isozymes I and II Streptococcus sp.
3.2.1.31 Cu2+ inhibition of isozymes I and II Streptococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 67000
-
2 * 67000, isozymes I and II, SDS-PAGE Streptococcus sp.
3.2.1.31 140000
-
isozymes I and II, gel filtration Streptococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Streptococcus sp.
-
isolated from human intestinal microflora, two isozymes I and II
-
3.2.1.31 Streptococcus sp. LJ-22
-
isolated from human intestinal microflora, two isozymes I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31 native isozymes I and II to homogeneity by ammonium sulfate fractionation, hydrophobic interaction, ion exchange, and hydroxyapatite chromatographies, and glycyrrhizin affinity chromatography Streptococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 0.137
-
purified isozyme I Streptococcus sp.
3.2.1.31 0.19
-
purified isozyme II Streptococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 glycyrrhizin + H2O specific substrate of isozymes I and II Streptococcus sp. 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
-
?
3.2.1.31 glycyrrhizin + H2O specific substrate of isozymes I and II Streptococcus sp. LJ-22 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
-
?
3.2.1.31 additional information no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide Streptococcus sp. ?
-
?
3.2.1.31 additional information no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide Streptococcus sp. LJ-22 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.31 dimer 2 * 67000, isozymes I and II, SDS-PAGE Streptococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 6.5
-
purified isozyme II Streptococcus sp.
3.2.1.31 7
-
purified isozyme I Streptococcus sp.