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Literature summary extracted from

  • Park, S.; Kim, J.; Kim, D.
    Purification and characterization of quercitrin-hydrolyzing alpha-L-rhamnosidase from Fusobacterium K-60, a human intestinal bacterium (2005), J. Microbiol. Biotechnol., 15, 519-524.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 4-Chloromercuriphenylsulfonate strong inhibition Fusobacterium sp.
3.2.1.40 Cu2+ strong inhibition Fusobacterium sp.
3.2.1.40 L-rhamnose strong product inhibition Fusobacterium sp.
3.2.1.40 Mn2+ strong inhibition Fusobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.40 0.057
-
 4-nitrophenyl alpha-L-rhamnopyranoside pH 5.5-7.0 Fusobacterium sp.
3.2.1.40 0.077
-
 quercitrin pH 5.5-7.0 Fusobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.40 41000
-
 4 * 41000, SDS-PAGE Fusobacterium sp.
3.2.1.40 170000
-
 gel filtration Fusobacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.40 quercitrin + H2O Fusobacterium sp.
-
 quercetin + L-rhamnose
-
 ?
3.2.1.40 quercitrin + H2O Fusobacterium sp. K-60
-
 quercetin + L-rhamnose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Fusobacterium sp.
-
 isolated from human intestine
-
3.2.1.40 Fusobacterium sp. K-60
-
 isolated from human intestine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 to homogeneity Fusobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 2.89
-
 purified enzyme Fusobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Fusobacterium sp. 4-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Fusobacterium sp. K-60 4-nitrophenol + alpha-L-rhamnose
-
 ?
3.2.1.40 quercitrin + H2O
-
 Fusobacterium sp. quercetin + L-rhamnose
-
 ?
3.2.1.40 quercitrin + H2O
-
 Fusobacterium sp. K-60 quercetin + L-rhamnose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.40 tetramer 4 * 41000, SDS-PAGE Fusobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 5.5 7
-
 Fusobacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.40 Fusobacterium sp.
-
-
 5.2