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Literature summary extracted from

  • Kontkanen, H.; Tenkanen, M.; Fagerstrom, R.; Reinikainen, T.
    Characterisation of steryl esterase activities in commercial lipase preparations (2004), J. Biotechnol., 108, 51-59.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Chromobacterium viscosum
-
enzyme activity in commercial lipase preparation from Asahi, comparison of different preparations
-
3.1.1.13 Diutina rugosa
-
enzyme activity in commercial lipase preparation from Amano, comparison of different preparations
-
3.1.1.13 Pseudomonas sp.
-
enzyme activity in commercial lipase preparation from Amano, comparison of different preparations
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 0.024
-
substrate cholesteryl stearate, pH 7.0 Pseudomonas sp.
3.1.1.13 0.12
-
substrate cholesteryl oleate, pH 7.0 Pseudomonas sp.
3.1.1.13 0.18
-
substrate cholesteryl linoleate, pH 7.0 Pseudomonas sp.
3.1.1.13 0.18
-
substrate cholesteryl linolenate, pH 7.0 Pseudomonas sp.
3.1.1.13 1.2
-
substrate cholesteryl stearate, pH 7.0 Diutina rugosa
3.1.1.13 1.98
-
substrate cholesteryl stearate, pH 7.0 Chromobacterium viscosum
3.1.1.13 3.1
-
substrate cholesteryl palmitate, pH 7.0 Pseudomonas sp.
3.1.1.13 4.6
-
substrate cholesteryl linoleate, pH 7.0 Chromobacterium viscosum
3.1.1.13 4.6
-
substrate cholesteryl linolenate, pH 7.0 Chromobacterium viscosum
3.1.1.13 4.8
-
substrate cholesteryl oleate, pH 7.0 Chromobacterium viscosum
3.1.1.13 5
-
substrate cholesteryl palmitate, pH 7.0 Diutina rugosa
3.1.1.13 6.9
-
substrate cholesteryl linoleate, pH 7.0 Diutina rugosa
3.1.1.13 7
-
substrate cholesteryl oleate, pH 7.0 Diutina rugosa
3.1.1.13 7.8
-
substrate cholesteryl palmitate, pH 7.0 Chromobacterium viscosum
3.1.1.13 11
-
substrate cholesteryl linolenate, pH 7.0 Diutina rugosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesteryl linoleate + H2O
-
Pseudomonas sp. cholesterol + linoleic acid
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Diutina rugosa cholesterol + linoleic acid
-
?
3.1.1.13 cholesteryl linoleate + H2O
-
Chromobacterium viscosum cholesterol + linoleic acid
-
?
3.1.1.13 cholesteryl linolenate + H2O
-
Pseudomonas sp. cholesterol + linolenic acid
-
?
3.1.1.13 cholesteryl linolenate + H2O
-
Diutina rugosa cholesterol + linolenic acid
-
?
3.1.1.13 cholesteryl linolenate + H2O
-
Chromobacterium viscosum cholesterol + linolenic acid
-
?
3.1.1.13 cholesteryl oleate + H2O
-
Pseudomonas sp. cholesterol + oleic acid
-
?
3.1.1.13 cholesteryl oleate + H2O
-
Diutina rugosa cholesterol + oleic acid
-
?
3.1.1.13 cholesteryl oleate + H2O
-
Chromobacterium viscosum cholesterol + oleic acid
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Pseudomonas sp. cholesterol + palmitic acid
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Diutina rugosa cholesterol + palmitic acid
-
?
3.1.1.13 cholesteryl palmitate + H2O
-
Chromobacterium viscosum cholesterol + palmitic acid
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Pseudomonas sp. cholesterol + stearic acid
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Diutina rugosa cholesterol + stearic acid
-
?
3.1.1.13 cholesteryl stearate + H2O
-
Chromobacterium viscosum cholesterol + stearic acid
-
?
3.1.1.13 additional information in aqueous dispersion, reaction proceeds slowly. Addition of surfactant Thesit accelerates initial reaction rate Diutina rugosa ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.13 60
-
-
Diutina rugosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.13 30 70 over 80% of maximum activity within this range Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.13 50
-
3 h, 80% residual activity Pseudomonas sp.
3.1.1.13 50
-
3 h, 80% residual activity Diutina rugosa