Any feedback?
Literature summary extracted from
- Novel tumor necrosis factor-responsive mammalian neutral sphingomyelinase-3 is a C-tail-anchored protein (2006), J. Biol. Chem., 281, 13784-13793.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | additional information | tumor necrosis factor stimulates rapid activation of nSMase3 in MCF7 cells with peak activity at 1.5 min | Homo sapiens | |
| 3.1.4.12 | phosphatidylserine | enhances activity | Homo sapiens |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | overexpression in Saccharomyces cerevisiae strain JK9-3dDELTAIsc1p, lacking endogenous SMase activity | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | scyphostatin | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.12 | 0.01871 | - |
sphingomyelin | - |
Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.12 | endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| 3.1.4.12 | Golgi apparatus | - |
Homo sapiens | 5794 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Mg2+ | 5 mM, stimulates | Homo sapiens | |
| 3.1.4.12 | Mn2+ | 5 mM, stimulates | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Homo sapiens | Q9NXE4 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | MCF-7 cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | sphingomyelin + H2O | - |
Homo sapiens | N-acylsphingosine + choline phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | neutral sphingomyelinase-3 | - |
Homo sapiens |
| 3.1.4.12 | nSMase3 | - |
Homo sapiens |
| 3.1.4.12 | SMPD4 | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 7 | - |
- |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
