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Literature summary extracted from

  • Hata, S.; Koyama, S.; Kawahara, H.; Doi, N.; Maeda, T.; Toyama-Sorimachi, N.; Abe, K.; Suzuki, K.; Sorimachi, H.
    Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP (2006), J. Biol. Chem., 281, 11214-11224.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.B28 Ca2+
-
 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.B28 expression in Escherichia coli Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B28 calpain inhibitor I 0.010 mM Mus musculus
3.4.22.B28 calpeptin 0.010 mM Mus musculus
3.4.22.B28 E64c 0.050 mM Mus musculus
3.4.22.B28 MG132 0.010 mM Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B28 cell surface mucous cells Rattus norvegicus 9986
-
3.4.22.B28 cell surface mucous cells Mus musculus 9986
-
3.4.22.B28 Golgi apparatus COS7 cells Rattus norvegicus 5794
-
3.4.22.B28 Golgi apparatus COS7 cells Mus musculus 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.53 Ca2+ dependent on Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.53 additional information Mus musculus functions for nCL-2 involve the membrane trafficking of mucus cells by interacting with coat proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B28 Mus musculus
-
-
-
3.4.22.B28 Rattus norvegicus
-
-
-
3.4.22.53 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.B28 recombinant protein from Escherichia coli Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.B28 duodenum
-
 Rattus norvegicus
-
3.4.22.B28 duodenum
-
 Mus musculus
-
3.4.22.B28 gastric mucosa in the gastric epithelium Rattus norvegicus
-
3.4.22.B28 gastric mucosa in the gastric epithelium Mus musculus
-
3.4.22.B28 intestinal mucosa mucous-secreting goblet cell in the duodenum Rattus norvegicus
-
3.4.22.B28 intestinal mucosa mucus-secreting goblet cell in the duodenum Mus musculus
-
3.4.22.B28 stomach
-
 Rattus norvegicus
-
3.4.22.B28 stomach
-
 Mus musculus
-
3.4.22.53 duodenal mucosa nCL-2 is localized strictly to the surface cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum Mus musculus
-
3.4.22.53 goblet cell nCL-2 is localized strictly to the surface cells in the gastric epithelium and the mucus-secreting goblet cells in the duodenum Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B28 beta-subunit of the coat protein + H2O 30°C, 5 mM Ca2+ Mus musculus ?
-
 ?
3.4.22.53 beta-subunit of coatomer complex beta-COP + H2O
-
 Mus musculus ?
-
 ?
3.4.22.53 additional information functions for nCL-2 involve the membrane trafficking of mucus cells by interacting with coat proteins Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.B28 monomer two-hybrid assay Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B28 calpain
-
 Rattus norvegicus
3.4.22.B28 calpain
-
 Mus musculus
3.4.22.B28 calpain-8a
-
 Rattus norvegicus
3.4.22.B28 calpain-8a
-
 Mus musculus
3.4.22.B28 nCL-2
-
 Rattus norvegicus
3.4.22.B28 nCL-2
-
 Mus musculus
3.4.22.53 nCL-2
-
 Mus musculus