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Literature summary extracted from

  • Mou, T.C.; Gille, A.; Fancy, D.A.; Seifert, R.; Sprang, S.R.
    Structural basis for the inhibition of mammalian membrane adenylyl cyclase by 2 '(3')-O-(N-methylanthraniloyl)-guanosine 5 '-triphosphate (2005), J. Biol. Chem., 280, 7253-7261.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.1 2.8 A and 2.9 A resolutions, crystal structures of the complex between stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate and the catalytic C1 and C2 domains from type V and type II adenylate cyclase, bound to forskolin and either 2'(3')-O-(N-methylanthraniloyl)-guanosine 5' triphosphate Mg2+ or 2'(3')-O-(N-methylanthraniloyl)-guanosine 5' triphosphate Mn2+ Mammalia

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.1 2'-d-(3')-O-(N-methylanthraniloyl)-GTP determination of Ki values under various experimental conditions Mammalia
4.6.1.1 2'-O-(N-methylanthraniloyl)-3'-deoxy-GTP determination of Ki values under various experimental conditions Mammalia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.1 0.4
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+ Mammalia
4.6.1.1 0.4
-
 ATP mutant VC1-A406P+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin Mammalia
4.6.1.1 0.43
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+, 0.1 mM forskolin, stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate Mammalia
4.6.1.1 0.43
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin Mammalia
4.6.1.1 0.53
-
 ATP mutant VC1-A409P+IIC2, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin Mammalia
4.6.1.1 0.55
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+, stimulatory G protein ALPHA subunits guanosine 5'-(gamma-thio)triphosphate Mammalia
4.6.1.1 0.56
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, 0.1 mM forskolin Mammalia
4.6.1.1 0.57
-
 ATP mutant VC1+IIC2-I1006V, 30°C, pH 7.4, 10 mM MnCl, 0.1 mM forskolin Mammalia
4.6.1.1 0.62
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mn2+, 0.1 mM forskolin Mammalia
4.6.1.1 0.68
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+ Mammalia
4.6.1.1 0.76
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate Mammalia
4.6.1.1 0.85
-
 ATP recombinant form VC1-IIC2, 30°C, pH 7.4, 10 mM Mg2+, 0.1 mM forskolin, stimulatory G protein alpha subunits guanosine 5'-(gamma-thio)triphosphate Mammalia

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Mammalia
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Mammalia cAMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 adenylyl cyclase
-
 Mammalia