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Literature summary extracted from

  • Odintsov, S.G.; Sabala, I.; Bourenkov, G.; Rybin, V.; Bochtler, M.
    Staphylococcus aureus aminopeptidase S is a founding member of a new peptidase clan (2005), J. Biol. Chem., 280, 27792-27799.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.24 expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.24 purified recombinant enzyme, sitting drop vapour diffusion method, the reservoir solution contains 0.1 M HEPES/NaOH, pH 7.6, 2.0 M ammonium sulfate, and 2% PEG 400, 4 weeks, X-ray diffraction structure determination and analysis at 1.8 A resolution Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.24 additional information the enzyme activity is completely abolished by metal chelating agents, but can be restored by addition of zinc or cobalt Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Co2+ the enzyme contains two metal ions with high occupancy and a third metal ion with low occupancy at the active site of the enzyme molecule, Glu319 and a water molecule are bridging, Glu253, His348, His381, Tyr355, and Asp383 are involved in metal binding, structure analysis Staphylococcus aureus
3.4.11.24 additional information determination of metal ion identity Staphylococcus aureus
3.4.11.24 Zn2+ the enzyme contains two metal ions with high occupancy and a third metal ion with low occupancy at the active site of the enzyme molecule, Glu319 and a water molecule are bridging, Glu253, His348, His381, Tyr355, and Asp383 are involved in metal binding, structure analysis Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Staphylococcus aureus
-
-
-
3.4.11.24 Staphylococcus aureus NCTC8325-4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.24 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 Met-enkephalin + H2O substrate is a pentapeptide with sequence Tyr-Gly-Gly-Phe-Met, stepwise degradation from the N-terminus Staphylococcus aureus ?
-
 ?
3.4.11.24 Met-enkephalin + H2O substrate is a pentapeptide with sequence Tyr-Gly-Gly-Phe-Met, stepwise degradation from the N-terminus Staphylococcus aureus NCTC8325-4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.24 dimer the enzyme forms an inactive dimer in the crystal with a large internal cavity with the active sites located at the opposing ends of the cavity essentially inaccessible from the outside Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 aminopeptidase S
-
 Staphylococcus aureus
3.4.11.24 AmpS
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.24 37
-
 assay at Staphylococcus aureus