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Literature summary extracted from
- Biochemical and molecular characterization of a novel choline-specific glycerophosphodiester phosphodiesterase belonging to the nucleotide pyrophosphatase/phosphodiesterase family (2005), J. Biol. Chem., 280, 23084-23093.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.4.3 | hNPP6-ex | truncated form of hNPP6, cDNA for the extracellular domain of hNPP6, amino acids 1-421 | Homo sapiens |
| 3.1.4.3 | mNPP6-ex | truncated form of hNPP6, cDNA for the extracellular domain of mNPP6 with Myc tag at the carboxyl terminus | Mus musculus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.3 | EDTA | enzyme activity against 1-myristoyl-lysophosphatidylcholine, glycerophosphorylcholine, and p-nitrophenyl phosphorylcholine are readily inhibited by EDTA. Indicates that a divalent cation is essential for catalytic activity | Homo sapiens |  |
| 3.1.4.3 | EGTA | enzyme activity against 1-myristoyl-lysophosphatidylcholine, glycerophosphorylcholine, and p-nitrophenyl phosphorylcholine are readily inhibited by EGTA. Indicates that a divalent cation is essential for catalytic activity | Homo sapiens | |
| 3.1.4.3 | additional information | the catalytic activity of NPP6 toward p-nitrophenyl phosphorylcholine is partially inhibited by the divalent cations. Attempts are unsuccessful to recover the activity of EDTA treated NPP6 by adding various divalent cations | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.3 | 0.165 | - |
1-palmitoyl-lysophosphatidylcholine | Vmax 89 nmol/min mg | Homo sapiens | |
| 3.1.4.3 | 0.296 | - |
1-lauroyl-lysophosphatidylcholine | Vmax 463 nmol/min mg | Homo sapiens | |
| 3.1.4.3 | 0.344 | - |
Glycerophosphorylcholine | Vmax 484 nmol/min mg | Homo sapiens | |
| 3.1.4.3 | 0.434 | - |
1-linoleoyl-lysophosphatidylcholine | Vmax 285 nmol/min mg | Homo sapiens | |
| 3.1.4.3 | 0.44 | - |
1-myristoyl-lysophosphatidylcholine | Vmax 367 nmol/min mg | Homo sapiens | |
| 3.1.4.3 | 0.563 | - |
1-arachidonoyl-lysophosphatidylcholine | Vmax 470 nmol/min mg | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.4.3 | plasma membrane | - |
Homo sapiens | 5886 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.3 | additional information | none of the divalent cations tested (Ca2+, Mg2+, Zn2+, Ni2+, Co2+, Fe2+, and Cu2+) rescued the lost activity after treatment with EDTA, EGTA | Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 50000 | - |
Western blotting analysis using anti-hNPP6 monoclonal antibody | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.3 | additional information | Mus musculus | release nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives | ? | - |
? | |
| 3.1.4.3 | additional information | Homo sapiens | release of nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.3 | Homo sapiens | Q6UWR7 | - |
- |
| 3.1.4.3 | Mus musculus | Q8BGN3 | NPP6 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.3 | mono Q ion exchange chromatography column and eluted with a linear gradient of NaCl (0-2 M) using the deltaKTA system | Mus musculus |
| 3.1.4.3 | mono Q ion exchange chromatography column and eluted with a linear gradient of NaCl (0-2 M) using the deltaKTA system | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.4.3 | brain | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | brain | NPP6 mRNA is predominantly detected in | Homo sapiens | - |
| 3.1.4.3 | cerebellum | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | cerebrum | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | colon | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | heart | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | HeLa cell | - |
Homo sapiens | - |
| 3.1.4.3 | kidney | NPP6 mRNA is predominantly detected in | Homo sapiens | - |
| 3.1.4.3 | kidney | NPP6 mRNA is predominantly detected in kidney | Mus musculus | - |
| 3.1.4.3 | liver | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | liver | lesser expression of NPP6 | Homo sapiens | - |
| 3.1.4.3 | lung | - |
Homo sapiens | - |
| 3.1.4.3 | lung | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | additional information | no expression of NPP6 detectable in heart, stomach, placenta, intestine, and leukocyte | Homo sapiens | - |
| 3.1.4.3 | additional information | no expression of NPP6 detectable in Modulla oblongata and uterus | Mus musculus | - |
| 3.1.4.3 | muscle | lesser expression of NPP6 | Homo sapiens | - |
| 3.1.4.3 | nephron | segment-specific expression pattern of NPP6 in nephrons shown | Homo sapiens | - |
| 3.1.4.3 | ovary | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | small intestine | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | spleen | lesser expression of NPP6 | Mus musculus | - |
| 3.1.4.3 | testis | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.3 | 1-arachidonoyl-2-lysophosphatidylcholine + H2O | best substrate | Homo sapiens | 1-arachidonoyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | 1-arachidoyl-2-lysophosphatidylcholine + H2O | poor substrate | Homo sapiens | 1-arachidoyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | 1-lauroyl-lysophosphatidylcholine + H2O | potent substrate | Homo sapiens | 1-lauroyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | 1-linoleoyl-lysophosphatidylcholine + H2O | second best substrate | Homo sapiens | 1-linoleoyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | 1-myristoyl-lysophosphatidylcholine + H2O | potent substrate | Homo sapiens | 1-myristoyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | 1-oleoyl-lysophosphatidylcholine + H2O | - |
Homo sapiens | 1-oleoyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | 1-palmitoyl-lysophosphatidylcholine + H2O | - |
Homo sapiens | 1-palmitoyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | 1-stearoyl-lysophosphatidylcholine + H2O | poor substrate | Homo sapiens | 1-stearoyl-sn-glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | egg lysophosphatidylcholine + H2O | NPP6 is a choline-specific glycerophosphodiester phosphodiesterase. In marked difference to NPP2 (lysoPLD/autotaxin), which equally hydrolyzes lysophosphatidylcholine, 1-oleoyl lysophosphatidylethanolamine, 1-oleoyl lysophosphatidylserine, and porcine liver lysophosphatidylinositol to form lysophosphatidic acid, NPP6 hydrolyzes only lysophosphatidylcholine | Homo sapiens | monoacylglycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | glycerophosphorylcholine + H2O | enzyme hydrolyzes GPC efficiently, measurement of GDE activity | Homo sapiens | glycerol + phosphorylcholine | - |
? | |
| 3.1.4.3 | lysoPAF + H2O | lyso platelet activating factor, partially hydrolyzed | Homo sapiens | ? | - |
? | |
| 3.1.4.3 | lysophosphatidylcholine + H2O | - |
Mus musculus | ? | - |
? | |
| 3.1.4.3 | additional information | release nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives | Mus musculus | ? | - |
? | |
| 3.1.4.3 | additional information | release of nucleoside 5'-monophosphate from a variety of nucleotides and nucleotide derivatives | Homo sapiens | ? | - |
? | |
| 3.1.4.3 | additional information | recombinant NPP6 does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate (pNP-TMP) and bis(p-nitrophenyl) phosphate. NPP6 does not act on sphingomyelin (SM) | Homo sapiens | ? | - |
? | |
| 3.1.4.3 | p-nitrophenyl phenylphosphate + H2O | pNPPP, classical NPP substrate | Homo sapiens | p-nitrophenol + phenylphosphate | - |
? | |
| 3.1.4.3 | p-nitrophenyl phenylphosphate + H2O | pNPPP, classical NPP substrate | Mus musculus | ? | - |
? | |
| 3.1.4.3 | p-nitrophenyl phosphorylcholine + H2O | recombinant NPP6 efficiently hydrolyzes the classical substrate for phospholipase C | Mus musculus | ? | - |
? | |
| 3.1.4.3 | p-nitrophenyl phosphorylcholine + H2O | pNPPC, classical NPP substrate, recombinant NPP6 efficiently hydrolyzes the classical substrate for phospholipase C | Homo sapiens | p-nitrophenol + phosphorylcholine | - |
? | |
| 3.1.4.3 | p-nitrophenyl phosphorylcholine + H2O | pNPPC, classical NPP substrate | Mus musculus | ? + p-nitrophenol | - |
? | |
| 3.1.4.3 | platelet activating factor + H2O | PAF, partially hydrolyzed | Homo sapiens | ? | - |
? | |
| 3.1.4.3 | sphingosylphosphorylcholine + H2O | SPC, measurement of lysoPLC activity of NPP6 toward choline-containing phospholipids | Homo sapiens | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.3 | cholinespecific glycerophosphodiester phosphodiesterase | - |
Mus musculus |
| 3.1.4.3 | cholinespecific glycerophosphodiester phosphodiesterase | - |
Homo sapiens |
| 3.1.4.3 | hNPP6 | - |
Homo sapiens |
| 3.1.4.3 | mNPP6 | - |
Mus musculus |
| 3.1.4.3 | NPP6 | - |
Mus musculus |
| 3.1.4.3 | NPP6 | - |
Homo sapiens |
| 3.1.4.3 | nucleotide pyrophosphatase/phosphodiesterase | - |
Mus musculus |
| 3.1.4.3 | nucleotide pyrophosphatase/phosphodiesterase | - |
Homo sapiens |
| 3.1.4.3 | phospholipase C | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.3 | 8.5 | 9.5 | - |
Homo sapiens |
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