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Literature summary extracted from

  • Yamaguchi, Y.; Kuroki, T.; Yasuzawa, H.; Higashi, T.; Jin, W.; Kawanami, A.; Yamagata, Y.; Arakawa, Y.; Goto, M.; Kurosaki, H.
    Probing the role of Asp-120(81) of metallo-beta-lactamase (IMP-1) by site-directed mutagenesis, kinetic studies, and X-ray crystallography (2005), J. Biol. Chem., 280, 20824-20832.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expression in Escherichia coli Serratia marcescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.2.6 hanging-drop vapour diffusion method. Three-dimensional structures of D120A mutant and D120E mutant determined at 2.0 A and 3.0 A resolution, respectively Serratia marcescens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 D120A kcat/KM is up to 425000fold lower than the wild-type enzyme. Ki-value for phosphate is 450fold lower than wild-type value Serratia marcescens
3.5.2.6 D120E kcat/KM is up to 136000fold lower than the wild-type enzyme. the Zn-Zn distance is increased by 0.3 A compared with wild-type enzyme Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 phosphate competitive inhibition. 50 mM, 30% decrease of wild-type activity. 5 mM, 90% decrease of activity of mutant enzyme D120A Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0012
-
 nitrocefin mutant enzyme D120E Serratia marcescens
3.5.2.6 0.0013
-
 nitrocefin mutant enzyme D120A Serratia marcescens
3.5.2.6 0.008
-
 cephaloridine wild-type enzyme Serratia marcescens
3.5.2.6 0.02
-
 nitrocefin wild-type enzyme Serratia marcescens
3.5.2.6 0.027
-
 nitrocefin mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 0.045
-
 Imipenem wild-type enzyme Serratia marcescens
3.5.2.6 0.05
-
 nitrocefin mutant enzyme D120E, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 0.08
-
 benzylpenicillin wild-type enzyme Serratia marcescens
3.5.2.6 0.15
-
 cephaloridine mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 0.2
-
 cephaloridine mutant enzyme D120E Serratia marcescens
3.5.2.6 0.2
-
 cephaloridine mutant enzyme D120E, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 0.27
-
 cephaloridine mutant enzyme D120A Serratia marcescens
3.5.2.6 0.64
-
 Imipenem mutant enzyme D120A Serratia marcescens
3.5.2.6 0.75
-
 Imipenem mutant enzyme D120E Serratia marcescens
3.5.2.6 2.6
-
 benzylpenicillin mutant enzyme D120A Serratia marcescens
3.5.2.6 3.1
-
 Imipenem mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 8
-
 benzylpenicillin mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zinc the Zn-Zn distance in D120E mutant is increased by 0.3 A compared with wild-type enzyme Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Serratia marcescens P52699
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 benzylpenicillin + H2O
-
 Serratia marcescens (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Serratia marcescens (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 imipenem + H2O
-
 Serratia marcescens (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Serratia marcescens (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 IMP-1
-
 Serratia marcescens
3.5.2.6 metallo-beta-lactamase IMP-1
-
 Serratia marcescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.0035
-
 cephaloridine mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 0.022
-
 cephaloridine mutant enzyme D120E, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 0.17
-
 benzylpenicillin mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 0.22
-
 cephaloridine mutant enzyme D120E Serratia marcescens
3.5.2.6 0.23
-
 cephaloridine mutant enzyme D120A Serratia marcescens
3.5.2.6 0.27
-
 nitrocefin mutant enzyme D120A Serratia marcescens
3.5.2.6 0.53
-
 benzylpenicillin mutant enzyme D120A Serratia marcescens
3.5.2.6 0.55
-
 nitrocefin mutant enzyme D120E Serratia marcescens
3.5.2.6 2.03
-
 Imipenem mutant enzyme D120A Serratia marcescens
3.5.2.6 3.17
-
 Imipenem mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 3.7
-
 Imipenem mutant enzyme D120E Serratia marcescens
3.5.2.6 4.17
-
 nitrocefin mutant enzyme D120E, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 7.5
-
 nitrocefin mutant enzyme D120A, 1 mM Zn(NO3)2 added Serratia marcescens
3.5.2.6 90
-
 cephaloridine wild-type enzyme Serratia marcescens
3.5.2.6 123.3
-
 Imipenem wild-type enzyme Serratia marcescens
3.5.2.6 466.7
-
 nitrocefin wild-type enzyme Serratia marcescens
3.5.2.6 535
-
 nitrocefin wild-type enzyme Serratia marcescens
3.5.2.6 650
-
 benzylpenicillin wild-type enzyme Serratia marcescens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 0.00032
-
 phosphate mutant enzyme D120A Serratia marcescens
3.5.2.6 0.144
-
 phosphate wild-type value Serratia marcescens