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Literature summary extracted from

  • Marraffini, L.A.; Ton-That, H.; Zong, Y.; Narayana, S.V.; Schneewind, O.
    Anchoring of surface proteins to the cell wall of Staphylococcus aureus. A conserved arginine residue is required for efficient catalysis of sortase A (2004), J. Biol. Chem., 279, 37763-37770.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.70 N98A kcat/Km is 1.2fold lower than wild-type value Staphylococcus aureus
3.4.22.70 N98Q kcat/Km is 1.1fold higher than wild-type value Staphylococcus aureus
3.4.22.70 R197A kcat/Km is 2769fold lower than wild-type value Staphylococcus aureus
3.4.22.70 R197H kcat/Km is 610fold lower than wild-type value Staphylococcus aureus
3.4.22.70 R197K kcat/Km is 2571fold lower than wild-type value Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.70 0.00824
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, wild-type enzyme Staphylococcus aureus
3.4.22.70 0.00932
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme N98Q Staphylococcus aureus
3.4.22.70 0.0097
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme N98A Staphylococcus aureus
3.4.22.70 0.01391
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme R197K Staphylococcus aureus
3.4.22.70 0.01597
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme R197A Staphylococcus aureus
3.4.22.70 0.0168
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme R197H Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.70 Abz-LPETG-Dap(Dnp) + H2O
-
 Staphylococcus aureus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.70 0.00019
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme R197K Staphylococcus aureus
3.4.22.70 0.00021
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme R197A Staphylococcus aureus
3.4.22.70 0.00098
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme R197H Staphylococcus aureus
3.4.22.70 0.29
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme N98A Staphylococcus aureus
3.4.22.70 0.3
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, wild-type enzyme Staphylococcus aureus
3.4.22.70 0.37
-
 Abz-LPETG-Dap(Dnp) 37°C, pH 7.5, mutant enzyme N98Q Staphylococcus aureus