Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nagy, T.; Nurizzo, D.; Davies, G.J.; Biely, P.; Lakey, J.H.; Bolam, D.N.; Gilbert, H.J.
    The alpha-glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants (2003), J. Biol. Chem., 278, 20286-20292.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.139 crystal structure of mutant E292A in complex with its substrate aldobiouronic acid Cellvibrio japonicus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.139 E292A inactive mutant enzyme Cellvibrio japonicus
3.2.1.139 K288A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.003% of wild-type value Cellvibrio japonicus
3.2.1.139 K360A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.019% of wild-type value Cellvibrio japonicus
3.2.1.139 R325A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.000096% of wild-type value Cellvibrio japonicus
3.2.1.139 V210A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 38% of wild-type value Cellvibrio japonicus
3.2.1.139 V210G kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is identical to wild-type value Cellvibrio japonicus
3.2.1.139 V210N kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 9.4% of wild-type value Cellvibrio japonicus
3.2.1.139 V210S kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 4.3% of wild-type value Cellvibrio japonicus
3.2.1.139 W160A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.0064% of wild-type value Cellvibrio japonicus
3.2.1.139 W543A kcat/KM for the substrate 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside is 0.069% of wild-type value Cellvibrio japonicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.139 4-O-Methyl-D-glucuronic acid
-
 Cellvibrio japonicus
3.2.1.139 D-glucose
-
 Cellvibrio japonicus
3.2.1.139 D-glucuronic acid
-
 Cellvibrio japonicus
3.2.1.139 D-xylose
-
 Cellvibrio japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.139 0.19
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210G Cellvibrio japonicus
3.2.1.139 0.21
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
3.2.1.139 0.69
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K288A Cellvibrio japonicus
3.2.1.139 0.82
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W543A Cellvibrio japonicus
3.2.1.139 0.86
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210A Cellvibrio japonicus
3.2.1.139 1.6
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K360A Cellvibrio japonicus
3.2.1.139 2.1
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W160A Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.139 Cellvibrio japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.139
-
 Cellvibrio japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.139 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside + H2O
-
 Cellvibrio japonicus 4-nitrophenyl-D-xylopyranoside + 4-O-methyl-alpha-D-glucuronate
-
 ?
3.2.1.139 aldobiouronic acid + H2O
-
 Cellvibrio japonicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.139 GlcA67A
-
 Cellvibrio japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.139 0.014
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K288A Cellvibrio japonicus
3.2.1.139 0.09
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W160A Cellvibrio japonicus
3.2.1.139 0.2
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme K360A Cellvibrio japonicus
3.2.1.139 0.38
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme W543A Cellvibrio japonicus
3.2.1.139 127
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210G Cellvibrio japonicus
3.2.1.139 143
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
3.2.1.139 220
-
 4-nitrophenyl-2-O-(4-O-methyl-alpha-D-glucuronosyl)-beta-D-xylopyranoside 37°C, pH 7.0, mutant enzyme V210A Cellvibrio japonicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.139 0.54
-
 4-O-methyl-glucuronic acid 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
3.2.1.139 3.84
-
 glucuronic acid 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
3.2.1.139 64.3
-
 glucuronic acid 37°C, pH 7.0, mutant enzyme V210N Cellvibrio japonicus
3.2.1.139 69.5
-
 xylose 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
3.2.1.139 77.1
-
 glucose 37°C, pH 7.0, wild-type enzyme Cellvibrio japonicus
3.2.1.139 78.3
-
 glucuronic acid 37°C, pH 7.0, mutant enzyme V210S Cellvibrio japonicus