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Literature summary extracted from

  • Heath, R.J.; Rock, C.O.
    Inhibition of beta-ketoacyl-acyl carrier protein synthase III (FabH) by acyl-acyl carrier protein in Escherichia coli (1996), J. Biol. Chem., 271, 10996-11000.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 expression of the His-tagged enzyme in strain BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.180 acyl-[acyl-carrier protein] mixed inhibition type, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein], inhibitory potency increases with increasing chain length between C12 and C20 Escherichia coli
2.3.1.180 additional information no inhibition by non-esterified [acyl-carrier-protein] Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.180 0.005
-
 malonyl-[acyl-carrier protein] pH 7.0, 37°C, recombinant enzyme Escherichia coli
2.3.1.180 0.04
-
 acetyl-CoA pH 7.0, 37°C, recombinant enzyme Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Escherichia coli initial condensation reaction to start fatty acid biosynthesis by the type II fatty acid synthase, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein] acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Escherichia coli
-
 gene fabH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 recombinant His-tagged enzyme from strain BL21(DE3) Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 0.004
-
 purified His-tagged recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] initial condensation reaction to start fatty acid biosynthesis by the type II fatty acid synthase, the enzyme is a target for fatty acid synthesis regulation by acyl-[acyl-carrier-protein] Escherichia coli acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] acetyl-CoA and propionyl-CoA are the preferred primers Escherichia coli acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 butyryl-CoA + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxo-hexanoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information no activity with hexanoyl-CoA or octanoyl-CoA Escherichia coli ?
-
 ?
2.3.1.180 propionyl-CoA + malonyl-[acyl-carrier protein] acetyl-CoA and propionyl-CoA are the preferred primers Escherichia coli 3-oxo-valeryl-[acyl-carrier protein] + CoA + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Escherichia coli
2.3.1.180 FabH
-
 Escherichia coli
2.3.1.180 [acyl-carrier-protein] synthase III
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.180 additional information
-
 additional information inhibition kinetics Escherichia coli