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Literature summary extracted from

  • Friedberg, E.C.; Hadi, S.M.; Goldthwait, D.A.
    Endonuclease II of Escherichia coli. II. Enzyme properties and studies on the degradation of alkylated and native deoxyribonucleic acid (1969), J. Biol. Chem., 244, 5879-5889.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.21.2 bovine serum albumin 130% activity at 1% at pH 8.0 and 37°C Escherichia coli
3.1.21.2 additional information the enzyme is induced by phage T4, no activation by S-adenosyl-L-methionine, ATP, or both Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.21.2 additional information analysis of enzyme activity in naturally occurring mutants of Escherichia coli compared to wild-type strain enzymes, overview Escherichia coli

General Stability

EC Number General Stability Organism
3.1.21.2 glycerol and bovine serum albumin stabilize Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.2 4-Chloromercuriphenylsulfonate
-
Escherichia coli
3.1.21.2 8-hydroxyquinoline inhibits cleavage of alkylated dsDNA Escherichia coli
3.1.21.2 Co2+
-
Escherichia coli
3.1.21.2 diethyldithiocarbamic acid
-
Escherichia coli
3.1.21.2 EDTA inhibits cleavage of non-alkylated dsDNA, 70% inhibition at 0.1 mM Escherichia coli
3.1.21.2 KCl 24% inhibition at 0.1 M, 42% at 0.2 M, and 64% at 0.3 M Escherichia coli
3.1.21.2 additional information enzyme inhibition by chelating and sulfhydryl agents, but not by tRNA and caffeine, DTT or 2-mercaptoethanol protect against inhibition by sulfhydryl reagents Escherichia coli
3.1.21.2 Zn2+
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.21.2 additional information
-
additional information kinetics Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.2 Mg2+ stimulates Escherichia coli
3.1.21.2 Mn2+ stimulates Escherichia coli
3.1.21.2 additional information the enzyme shows no absolute requirement for divalent cations, no effect by Ca2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.2 additional information
-
sucrose density gradient sedimentation equilibrium analysis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.2 double-stranded alkylated DNA + H2O Escherichia coli endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides 5'-phosphooligonucleotides
-
ir
3.1.21.2 double-stranded nativeDNA + H2O Escherichia coli endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides 5'-phosphooligonucleotides
-
ir

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.2 Escherichia coli
-
several strains
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.2
-
Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.21.2 Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products reaction mechanism Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.2 additional information
-
relative activity in several Escherichia coli wild-type and mutant strains, overview Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.1.21.2 -20°C, purified enzyme, 40 mM potassium phosphate, pH 6.5, or 50 mM Tris-HCl, pH 8.0, with 0.1 mM DTT or 2-mercaptoethanol, moderately stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.2 double-stranded alkylated DNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides Escherichia coli 5'-phosphooligonucleotides
-
ir
3.1.21.2 double-stranded alkylated DNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, hydrolysis of a phosphodiester bond near an alkylated base in native DNA without single breaks in the region, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1 Escherichia coli 5'-phosphooligonucleotides
-
ir
3.1.21.2 double-stranded native DNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides, the enzyme makes predominantly single-strand break, and a few double-strand breaks, the ratio is 3.7:1 Escherichia coli 5'-phosphooligonucleotides
-
ir
3.1.21.2 double-stranded nativeDNA + H2O endonucleolytic cleavage of DNA into 5'-phosphooligonucleotides Escherichia coli 5'-phosphooligonucleotides
-
ir
3.1.21.2 additional information DNA substrate specificity, overview Escherichia coli ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.2 endonuclease II
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.2 37
-
assay at Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.21.2 37
-
loss of 41% activity after 30 min, loss of 92% activity after 2 h, with addition 50% glycerol only 6% activity is lost within 2 h Escherichia coli
3.1.21.2 45
-
10 min, fraction IV, loss of 10% activity Escherichia coli
3.1.21.2 60
-
10 min, fraction IV, complete inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.2 8 9
-
Escherichia coli