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Literature summary extracted from

  • Kim, W.S.; Kim, B.T.; et.al.
    Purification of heparin lyase I from Bacteroides stercoris HJ-15 (2004), J. Biochem. Mol. Biol., 37, 684-690.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.2.7 EDTA 0.1 mM, 104% increase in activity Bacteroides stercoris
4.2.2.7 iodoacetic acid 0.1 mM, 53% increase in activity Bacteroides stercoris

General Stability

EC Number General Stability Organism
4.2.2.7 enzyme is stabilized by dithiothreitol Bacteroides stercoris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.7 carbodiimide 0.1 mM, 27% inhibition Bacteroides stercoris
4.2.2.7 Cd2+ 0.1 mM, 22% inhibition Bacteroides stercoris
4.2.2.7 Mg2+ 0.1 mM, 17% inhibition Bacteroides stercoris
4.2.2.7 N-tosyl-L-lysine-chloromethyl ketone 0.1 mM, 84% inhibition Bacteroides stercoris
4.2.2.7 p-chloromercuriphenyl sulfonic acid 0.1 mM, complete inhibition Bacteroides stercoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.7 0.013
-
 heparin pH 7.0, 45°C, heparin from porcine mucosa Bacteroides stercoris
4.2.2.7 0.21
-
 heparan sulfate pH 7.0, 45°C, heparin from porcine mucosa Bacteroides stercoris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.7 Co2+ 0.1 mM, 123% increase in activity Bacteroides stercoris
4.2.2.7 Cu2+ 0.1 mM, 14% increase in activity Bacteroides stercoris
4.2.2.7 NaCl 500 mM, required for maximal activity Bacteroides stercoris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.7 48000
-
 x * 48000, SDS-PAGE Bacteroides stercoris

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.7 Bacteroides stercoris
-
-
-
4.2.2.7 Bacteroides stercoris HJ-15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.7
-
 Bacteroides stercoris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.7 intestine
-
 Bacteroides stercoris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.7 2-O-desulfated heparin activity is 26% of the activity with heparin Bacteroides stercoris ?
-
 ?
4.2.2.7 2-O-desulfated heparin activity is 26% of the activity with heparin Bacteroides stercoris HJ-15 ?
-
 ?
4.2.2.7 heparan sulfate enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin Bacteroides stercoris ?
-
 ?
4.2.2.7 heparan sulfate enzyme prefers heparin to heparan sulfate. Activity with heparan sulfate from porcine mucosa is 17% of the activity with heparin. Activity with heparan sulfate from bovine kidney is 15% of the activity with heparin Bacteroides stercoris HJ-15 ?
-
 ?
4.2.2.7 heparin enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) Bacteroides stercoris ?
-
 ?
4.2.2.7 heparin enzyme prefers heparin to heparan sulfate. The enzyme acts preferentially on the trisulfated sequence ->4-alpha-D-GlcNS6S(1->4)-alpha-L-IdoA2S1-> comprising heparin affording the unsaturated trisulfated disaccharid (DELTAUA2S-GlcNS6S) Bacteroides stercoris HJ-15 ?
-
 ?
4.2.2.7 additional information no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin Bacteroides stercoris ?
-
 ?
4.2.2.7 additional information no activity with acharan sulfate, chondroitin sulfate and N-desulfated heparin Bacteroides stercoris HJ-15 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.7 ? x * 48000, SDS-PAGE Bacteroides stercoris

Synonyms

EC Number Synonyms Comment Organism
4.2.2.7 heparin lyase I
-
 Bacteroides stercoris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.7 50
-
-
 Bacteroides stercoris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.7 30 56 30°C: about 40% of maximal activity, 56°C: about 650% of maximal activity Bacteroides stercoris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.7 7
-
-
 Bacteroides stercoris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.7 Bacteroides stercoris isoelectric focusing
-
 9