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Literature summary extracted from
- Unusual extra space at the active site and high activity for acetylated hydroxyproline of prolyl aminopeptidase from Serratia marcescens (2006), J. Bacteriol., 188, 1599-1606.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.5 | expressed in Escherichia coli | Serratia marcescens |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.11.5 | hanging drop vapor diffusion | Serratia marcescens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.5 | Ala-TBODA | 1 mM | Serratia marcescens |  |
| 3.4.11.5 | Sar-TBODA | 10 mM | Serratia marcescens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.5 | 0.42 | - |
Pro-2-naphthylamide | Y149A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.51 | - |
Pro-2-naphthylamide | wild type enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.83 | - |
L-hydroxyproline-2-naphthylamide | Pro-bNA, F139A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.84 | - |
hydroxyproline-beta-naphthylamide | F236A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.13 | - |
hydroxyproline-beta-naphthylamide | Y149A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.17 | - |
Pro-2-naphthylamide | F139A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.31 | - |
Pro-2-naphthylamide | F236A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.45 | - |
4-acetyloxyproline-beta-naphthylamide | F139A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.58 | - |
Pro-2-naphthylamide | C271A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.6 | - |
4-acetyloxyproline-beta-naphthylamide | F236A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.64 | - |
4-acetyloxyproline-beta-naphthylamide | wild type enzyme | Serratia marcescens | |
| 3.4.11.5 | 2.67 | - |
4-acetyloxyproline-beta-naphthylamide | C271A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 2.92 | - |
4-acetyloxyproline-beta-naphthylamide | Y149A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 4.27 | - |
hydroxyproline-beta-naphthylamide | C271A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 9.3 | - |
L-hydroxyproline-2-naphthylamide | wild type enzyme | Serratia marcescens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.5 | Serratia marcescens | O32449 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.5 | column chromatography | Serratia marcescens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.5 | 0.21 | - |
L-hydroxyproline-2-naphthylamide, at 37°C, pH 8.0 | Serratia marcescens |
| 3.4.11.5 | 0.8 | - |
Pro-2-naphthylamide, at 37°C, pH 8.0 | Serratia marcescens |
| 3.4.11.5 | 8.34 | - |
4-acetyloxyproline-2-naphthylamide, at 37°C, pH 8.0 | Serratia marcescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.5 | 4-acetyloxyproline-2-naphthylamide + H2O | - |
Serratia marcescens | 2-naphthylamine + 4-acetyloxyproline | - |
? | |
| 3.4.11.5 | L-hydroxyproline-2-naphthylamide + H2O | - |
Serratia marcescens | 2-naphthylamine + L-hydroxyproline | - |
? | |
| 3.4.11.5 | L-hydroxyprolyl-2-naphthylamide + H2O | - |
Serratia marcescens | L-hydroxyproline + 2-naphthylamine | - |
? | |
| 3.4.11.5 | Pro-2-naphthylamide + H2O | - |
Serratia marcescens | beta-naphthylamine + L-proline | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.5 | proline iminopeptidase | - |
Serratia marcescens |
| 3.4.11.5 | Prolyl aminopeptidase | - |
Serratia marcescens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.5 | 0.017 | - |
L-hydroxyproline-2-naphthylamide | F236A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.03 | 0.55 | Pro-2-naphthylamide | C271A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.063 | - |
L-hydroxyproline-2-naphthylamide | F139A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.12 | - |
L-hydroxyproline-2-naphthylamide | Y149A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.35 | - |
Pro-2-naphthylamide | F236A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.63 | - |
Pro-2-naphthylamide | Y149A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.69 | - |
Pro-2-naphthylamide | F139A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 0.88 | - |
4-acetyloxyproline-2-naphthylamide | Y149A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 1.79 | - |
L-hydroxyproline-2-naphthylamide | C271A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 3.13 | - |
4-acetyloxyproline-2-naphthylamide | F236A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 3.52 | - |
4-acetyloxyproline-2-naphthylamide | F236A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 3.9 | - |
L-hydroxyproline-2-naphthylamide | wild type enzyme | Serratia marcescens | |
| 3.4.11.5 | 4.93 | - |
4-acetyloxyproline-2-naphthylamide | C271A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 5.85 | - |
4-acetyloxyproline-2-naphthylamide | F139A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 7.09 | - |
Pro-2-naphthylamide | C271A mutant enzyme | Serratia marcescens | |
| 3.4.11.5 | 9.7 | - |
Pro-2-naphthylamide | wild type enzyme | Serratia marcescens | |
| 3.4.11.5 | 84.1 | - |
4-acetyloxyproline-2-naphthylamide | wild type enzyme | Serratia marcescens | |
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