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Literature summary extracted from

  • Meister, M.; Saum, S.; Alber, B.E.; Fuchs, G.
    L-Malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase is involved in acetate assimilation of the isocitrate lyase-negative bacterium Rhodobacter capsulatus (2005), J. Bacteriol., 187, 1415-1425.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.24 EDTA
-
 Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.24 0.015
-
 malyl-CoA
-
 Rhodobacter capsulatus
4.1.3.24 0.021
-
 erythro-beta-methylmalyl-CoA
-
 Rhodobacter capsulatus
4.1.3.24 0.14
-
 acetyl-CoA
-
 Rhodobacter capsulatus
4.1.3.24 1.2
-
 glyoxylate
-
 Rhodobacter capsulatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.24 Co2+ stimulates Rhodobacter capsulatus
4.1.3.24 Mg2+ stimulates Rhodobacter capsulatus
4.1.3.24 Mn2+ stimulation by divalent ions, preference for Mn2+ Rhodobacter capsulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.24 35000
-
 6 * 35000, SDS-PAGE Rhodobacter capsulatus
4.1.3.24 195000
-
 gel filtration Rhodobacter capsulatus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.24 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.24
-
 Rhodobacter capsulatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.24 culture condition:acetate-grown cell malyl-CoA lyase activity is 20fold upregulated in acetate-grown cells versus glucose-grown cells Rhodobacter capsulatus
-
4.1.3.24 culture condition:L-glucose-grown cell malyl-CoA lyase activity is 20fold upregulated in acetate-grown cells versus glucose-grown cells Rhodobacter capsulatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.24 5.7
-
 cleavage of erythro-beta-methylmalyl-CoA Rhodobacter capsulatus
4.1.3.24 18
-
 cleavage of L-malyl-CoA Rhodobacter capsulatus
4.1.3.24 37
-
 condensation of acetyl-CoA with glyoxylate Rhodobacter capsulatus

Storage Stability

EC Number Storage Stability Organism
4.1.3.24 -20°C, 10% w/w glycerol, stable for several weeks Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.24 acetyl-CoA + glyoxylate
-
 Rhodobacter capsulatus L-malyl-CoA
-
 r
4.1.3.24 propionyl-CoA + glyoxylate
-
 Rhodobacter capsulatus erythro-beta-methylmalyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.3.24 hexamer 6 * 35000, SDS-PAGE Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
4.1.3.24 L-malyl-coenzyme A/beta-methylmalyl-coenzyme A lyase
-
 Rhodobacter capsulatus