EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.132 | sitting drop vapor diffusion method at 20°C. The crystal structure of mutant K218P reveals that the main chain and side chain structures of the loop comprising Lys218 are effected by the mutation. It is concluded that the flexible loop comprising Lys128 plays an important role in substrate binding | Niallia circulans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.132 | D57A | mutant enzyme shows 0.5% of wild-type activity. Mutant enzyme D57A produces smaller amounts of chitobiose and chitotetraose as compared to chitotriose than does the wild-type enzyme | Streptomyces sp. |
3.2.1.132 | D57N | mutant enzyme shows 72% of wild-type activity | Streptomyces sp. |
3.2.1.132 | K218P | mutant enzyme shows 0.16% of wild-type activity with acetylated chitosan | Niallia circulans |
3.2.1.132 | Y148S | mutant enzyme shows 12% of wild-type activity with acetylated chitosan | Niallia circulans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.132 | Niallia circulans | - |
MH-K1 | - |
3.2.1.132 | Niallia circulans MH-K1 | - |
MH-K1 | - |
3.2.1.132 | Streptomyces sp. | - |
N174 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.132 | acetylated chitosan + H2O | - |
Niallia circulans | ? | - |
? | |
3.2.1.132 | acetylated chitosan + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.132 | acetylated chitosan + H2O | - |
Niallia circulans MH-K1 | ? | - |
? | |
3.2.1.132 | chitohexaose + H2O | - |
Streptomyces sp. | chitobiose + chitotriose + chitotetraose | mutant enzyme D57A produces smaller amounts of chitobiose and chitotetraose as compared to chitotriose than does the wild-type enzyme | ? | |
3.2.1.132 | chitopentaose + H2O | - |
Streptomyces sp. | ? | - |
? | |
3.2.1.132 | chitotetraose + H2O | - |
Streptomyces sp. | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.132 | MH-K1 chitosanase | - |
Niallia circulans |
3.2.1.132 | N174 chitosanase | - |
Streptomyces sp. |