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Literature summary extracted from

  • Fukamizo, T.; Yoshikawa, T.; Katsumi, T.; Amano, S.; Saito, J.; Miki, K.; Ando, A.; Brzezinski, R.
    Substrate-binding mode of bacterial chitosanases (2005), J. Appl. Glycosci., 52, 183-189.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.132 sitting drop vapor diffusion method at 20°C. The crystal structure of mutant K218P reveals that the main chain and side chain structures of the loop comprising Lys218 are effected by the mutation. It is concluded that the flexible loop comprising Lys128 plays an important role in substrate binding Niallia circulans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 D57A mutant enzyme shows 0.5% of wild-type activity. Mutant enzyme D57A produces smaller amounts of chitobiose and chitotetraose as compared to chitotriose than does the wild-type enzyme Streptomyces sp.
3.2.1.132 D57N mutant enzyme shows 72% of wild-type activity Streptomyces sp.
3.2.1.132 K218P mutant enzyme shows 0.16% of wild-type activity with acetylated chitosan Niallia circulans
3.2.1.132 Y148S mutant enzyme shows 12% of wild-type activity with acetylated chitosan Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Niallia circulans
-
MH-K1
-
3.2.1.132 Niallia circulans MH-K1
-
MH-K1
-
3.2.1.132 Streptomyces sp.
-
N174
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 acetylated chitosan + H2O
-
Niallia circulans ?
-
?
3.2.1.132 acetylated chitosan + H2O
-
Streptomyces sp. ?
-
?
3.2.1.132 acetylated chitosan + H2O
-
Niallia circulans MH-K1 ?
-
?
3.2.1.132 chitohexaose + H2O
-
Streptomyces sp. chitobiose + chitotriose + chitotetraose mutant enzyme D57A produces smaller amounts of chitobiose and chitotetraose as compared to chitotriose than does the wild-type enzyme ?
3.2.1.132 chitopentaose + H2O
-
Streptomyces sp. ?
-
?
3.2.1.132 chitotetraose + H2O
-
Streptomyces sp. ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 MH-K1 chitosanase
-
Niallia circulans
3.2.1.132 N174 chitosanase
-
Streptomyces sp.