EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.76 | DTT | 31% inhibition at 0.05 mM | Pseudomonas fluorescens | |
3.1.1.76 | DTT | 38% inhibition at 0.05 mM | Pseudomonas putida | |
3.1.1.76 | EDTA | 25% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
3.1.1.76 | KCN | 13% inhibition at 0.005 mM | Pseudomonas aeruginosa | |
3.1.1.76 | KCN | 14% inhibition at 0.005 mM | Pseudomonas putida | |
3.1.1.76 | additional information | no inhibition by DTT | Pseudomonas aeruginosa | |
3.1.1.76 | additional information | no inhibition by KCN, PMSF, sodium azide, EDTA, and sodium deoxycholate | Pseudomonas fluorescens | |
3.1.1.76 | additional information | no inhibition by sodium azide, EDTA, and sodium deoxycholate | Pseudomonas putida | |
3.1.1.76 | PMSF | 23% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
3.1.1.76 | PMSF | 33% inhibition at 0.05 mM | Pseudomonas putida | |
3.1.1.76 | Sodium azide | 30% inhibition at 0.05 mM | Pseudomonas aeruginosa | |
3.1.1.76 | sodium deoxycholate | 35% inhibition at 0.5 mM | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.76 | 2.8 | - |
poly(3-hydroxybutyrate) | pH 7.9, 30°C | Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.76 | Ca2+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
3.1.1.76 | K+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
3.1.1.76 | Mg2+ | stimulates at 1 mM | Pseudomonas aeruginosa | |
3.1.1.76 | additional information | the enzyme requires divalent metal cations for activity | Pseudomonas fluorescens | |
3.1.1.76 | additional information | the enzyme requires divalent metal cations for activity | Pseudomonas aeruginosa | |
3.1.1.76 | additional information | the enzyme requires divalent metal cations for activity | Pseudomonas putida | |
3.1.1.76 | Na+ | stimulates at 1 mM | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.76 | poly(3-hydroxybutyrate) + H2O | Pseudomonas fluorescens | - |
oligo(3-hydroxybutyrate) | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate) + H2O | Pseudomonas aeruginosa | - |
oligo(3-hydroxybutyrate) | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate) + H2O | Pseudomonas putida | - |
oligo(3-hydroxybutyrate) | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas fluorescens | co-polymeric substrate | ? | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas aeruginosa | co-polymeric substrate | ? | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | Pseudomonas putida | co-polymeric substrate | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.76 | Pseudomonas aeruginosa | - |
isolated from fuel-oil contaminated soil | - |
3.1.1.76 | Pseudomonas fluorescens | - |
isolated from fuel-oil contaminated soil | - |
3.1.1.76 | Pseudomonas putida | - |
isolated from fuel-oil contaminated soil | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | additional information | - |
- |
Pseudomonas fluorescens |
3.1.1.76 | additional information | - |
- |
Pseudomonas aeruginosa |
3.1.1.76 | additional information | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.76 | poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas fluorescens | oligo(3-hydroxybutyrate) | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas aeruginosa | oligo(3-hydroxybutyrate) | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate) + H2O | - |
Pseudomonas putida | oligo(3-hydroxybutyrate) | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas fluorescens | ? | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas aeruginosa | ? | - |
? | |
3.1.1.76 | poly(3-hydroxybutyrate-co-3-hydroxyvalerate) + H2O | co-polymeric substrate | Pseudomonas putida | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | 30 | - |
assay at | Pseudomonas fluorescens |
3.1.1.76 | 30 | - |
assay at | Pseudomonas aeruginosa |
3.1.1.76 | 30 | - |
assay at | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.76 | 7.9 | - |
assay at | Pseudomonas fluorescens |
3.1.1.76 | 7.9 | - |
assay at | Pseudomonas aeruginosa |
3.1.1.76 | 7.9 | - |
assay at | Pseudomonas putida |