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Literature summary extracted from
- Partial purification and characterization of alpha-amylase produced by Aspergillus oryzae using spent-brewing grains (2005), Indian J. Biotechnol., 4, 336-341.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Cu2+ | - |
Aspergillus oryzae |  |
| 3.2.1.1 | Hg2+ | - |
Aspergillus oryzae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ca2+ | stabilizes the partially purified enzyme against thermal inactivation | Aspergillus oryzae | |
| 3.2.1.1 | Fe2+ | activates | Aspergillus oryzae | |
| 3.2.1.1 | Mn2+ | activates | Aspergillus oryzae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 66000 | - |
x * 66000, SDS-PAGE | Aspergillus oryzae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Aspergillus oryzae | - |
IFO 30103 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | partial 2.4fold purification of native enzyme by ammonium sulfate fractionation | Aspergillus oryzae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | additional information | soli fermentation on spent-brewing grains | Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
enzyme activity in solid fermentation cultures grown on spent-brewing grains | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | starch + H2O | - |
Aspergillus oryzae | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 66000, SDS-PAGE | Aspergillus oryzae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 50 | - |
- |
Aspergillus oryzae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 50 | - |
partially purified enzyme, rapid loss of activity, Ca2+ protects | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5 | - |
- |
Aspergillus oryzae |
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Release 2023.1 (January 2023)
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