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Literature summary extracted from

  • Adang, M.J.
    Insect aminopeptidase N (2004), Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ), 1, 296-299.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 expression in Escherichia coli, which is more difficult for the full-length enzyme as for fragments, successfull expression of isozyme APN1 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection system, but not of isozyme APN2, transient expression of isozyme APN1 on the cell surface in Sf21 cells under control of the inducible Drosophila HSP70 promoter Plutella xylostella

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.2 additional information the slamdance mutant, sda, has a modification of the APN structural gene, mutant flies exhibit altered neuronal excitability and are prone to seizures Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline isozyme APN1 Bombyx mori
3.4.11.2 2,2'-dipyridyl isozyme APN1 Bombyx mori
3.4.11.2 actinonin isozyme APN4: IC50 is 0.148 mM, inhibits also isozyme APN1 Bombyx mori
3.4.11.2 amastatin isozyme APN4: IC50 is 0.0076 mM, inhibits also isozyme APN1 Bombyx mori
3.4.11.2 amastatin strong inhibition Tenebrio molitor
3.4.11.2 bestatin
-
 Tenebrio molitor
3.4.11.2 probestin isozyme APN4: IC50 is 0.074 mM, inhibits also isozyme APN1 Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 membrane brush border Manduca sexta 16020
-
3.4.11.2 membrane brush border Plutella xylostella 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Zn2+ metallopeptidase Bombyx mori
3.4.11.2 Zn2+ the enzyme possesses a central Zn2+ coordination site H1EXXHX18E24 Heliothis virescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.2 90000
-
-
 Tenebrio molitor
3.4.11.2 110000
-
 isozyme APN4 Bombyx mori
3.4.11.2 120000
-
 isozyme APN1 Bombyx mori
3.4.11.2 120000
-
 isozyme APN1 Epiphyas postvittana
3.4.11.2 120000 170000
-
 Heliothis virescens
3.4.11.2 130000
-
 recombinant isozyme APN1 from Sf9 cells Plutella xylostella

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 additional information Drosophila melanogaster the enzyme plays a role in behaviour and nervous system excitability ?
-
 ?
3.4.11.2 additional information Helicoverpa punctigera the isozymes have different roles in digestion ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Bombyx mori
-
-
-
3.4.11.2 Drosophila melanogaster
-
-
-
3.4.11.2 Epiphyas postvittana
-
-
-
3.4.11.2 Helicoverpa punctigera
-
 isozymes APN1-4
-
3.4.11.2 Heliothis virescens
-
-
-
3.4.11.2 Manduca sexta
-
-
-
3.4.11.2 Plutella xylostella
-
-
-
3.4.11.2 Tenebrio molitor
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.2 glycoprotein
-
 Tenebrio molitor
3.4.11.2 glycoprotein about 30% carbohydrate content, isozyme APN1 is rich in serine and threonine residues, potential O-glycosylation sites, near the C-terminus Heliothis virescens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2 by preparation of brush border membrane vesicles and wheat germ agglutinin affinity chromatography Heliothis virescens
3.4.11.2 by preparation of brush border membrane vesicles from whole larvae, anion exchange and hydrophobic interaction chromatography, or separation by affinity binding to Cry1Ac toxin Manduca sexta
3.4.11.2 partially by preparation of brush border membrane vesicles from whole larvae Plutella xylostella
3.4.11.2 separation by affinity binding of brush border membrane vesicle enzyme to Cry1Ac toxin Bombyx mori
3.4.11.2 to homogeneity Tenebrio molitor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 central nervous system
-
 Drosophila melanogaster
-
3.4.11.2 larva
-
 Manduca sexta
-
3.4.11.2 larva
-
 Plutella xylostella
-
3.4.11.2 larva expression of isozymes APN1 and APN2 during larval development in neostatestage and third larval stage and during the entire development, respectively Helicoverpa punctigera
-
3.4.11.2 midgut
-
 Manduca sexta
-
3.4.11.2 midgut
-
 Plutella xylostella
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 Gly-4-nitroanilide + H2O low activity Bombyx mori Gly + 4-nitroaniline
-
 ?
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Bombyx mori L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 L-Arg-4-nitroanilide + H2O
-
 Bombyx mori L-Arg + 4-nitroaniline
-
 ?
3.4.11.2 L-Leu-4-nitroanilide + H2O
-
 Bombyx mori L-Leu + 4-nitroaniline
-
 ?
3.4.11.2 L-Lys-4-nitroanilide + H2O
-
 Bombyx mori L-Lys + 4-nitroaniline
-
 ?
3.4.11.2 L-Met-4-nitroanilide + H2O preferred substrate Bombyx mori L-Met + 4-nitroaniline
-
 ?
3.4.11.2 L-Pro-4-nitroanilide + H2O low activity Bombyx mori L-Pro + 4-nitroaniline
-
 ?
3.4.11.2 L-Val-4-nitroanilide + H2O low activity Bombyx mori L-Val + 4-nitroaniline
-
 ?
3.4.11.2 additional information the enzyme plays a role in behaviour and nervous system excitability Drosophila melanogaster ?
-
 ?
3.4.11.2 additional information the isozymes have different roles in digestion Helicoverpa punctigera ?
-
 ?
3.4.11.2 additional information broad substrate specificity for N-terminal amino acid residues, no activity with Asp-2-naphthylamide, the enzyme prefers tri- and tetrapeptides to di- and pentapeptides Tenebrio molitor ?
-
 ?
3.4.11.2 additional information isozyme APN1 shows preference for leucine amides, but also hydrolyzes Ile, Trp, Phe, Val, and His amides, no activity with Ser, Pro, Ala, Gly, and ARg derivatives Epiphyas postvittana ?
-
 ?
3.4.11.2 additional information the enzyme binds the Cry1Ac toxin from Bacillus thuringiensis Manduca sexta ?
-
 ?
3.4.11.2 additional information the enzyme prefers substrates with N-terminal nonpolar amino acids, isoyzme APN1 binds the Cry1Ac toxin from Bacillus thuringiensis via an N-acetylglucosamine moiety Bombyx mori ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 More the enzyme has a C-terminal anchor, and the N-terminal motif Trp-Arg-Leu-Pro Tenebrio molitor

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 aminopeptidase N
-
 Drosophila melanogaster
3.4.11.2 aminopeptidase N
-
 Bombyx mori
3.4.11.2 aminopeptidase N
-
 Tenebrio molitor
3.4.11.2 aminopeptidase N
-
 Manduca sexta
3.4.11.2 aminopeptidase N
-
 Plutella xylostella
3.4.11.2 aminopeptidase N
-
 Heliothis virescens
3.4.11.2 aminopeptidase N
-
 Epiphyas postvittana
3.4.11.2 aminopeptidase N
-
 Helicoverpa punctigera
3.4.11.2 APN
-
 Drosophila melanogaster
3.4.11.2 APN
-
 Tenebrio molitor
3.4.11.2 APN
-
 Manduca sexta
3.4.11.2 APN
-
 Helicoverpa punctigera
3.4.11.2 APN1
-
 Bombyx mori
3.4.11.2 APN1
-
 Plutella xylostella
3.4.11.2 APN1
-
 Heliothis virescens
3.4.11.2 APN1
-
 Epiphyas postvittana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 8
-
-
 Tenebrio molitor

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.11.2 Tenebrio molitor
-
-
 7.4

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.2 0.0076
-
 isozyme APN4: IC50 is 0.0076 mM, inhibits also isozyme APN1 Bombyx mori amastatin
3.4.11.2 0.074
-
 isozyme APN4: IC50 is 0.074 mM, inhibits also isozyme APN1 Bombyx mori probestin
3.4.11.2 0.148
-
 isozyme APN4: IC50 is 0.148 mM, inhibits also isozyme APN1 Bombyx mori actinonin