EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | additional information | wounding induces LAP-A | Solanum tuberosum | |
3.4.11.1 | additional information | wounding induces LAP-A, which is also induced by salt- and water-stress, and pathogen attack | Solanum lycopersicum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.1 | DNA sequence determination, overexpression of a LAP fusion protein in Escherichia coli | Arabidopsis thaliana |
3.4.11.1 | DNA sequence determination, overexpression of mature LAP-A, His6-tagged LAP-A and pre-LAP-A in Escherichia coli | Solanum lycopersicum |
3.4.11.1 | DNA sequence determination, overexpression of pre-LAP protein in Escherichia coli | Solanum tuberosum |
3.4.11.1 | overexpression in Escherichia coli | Phaseolus vulgaris |
3.4.11.1 | overexpression in Escherichia coli | Hordeum vulgare |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.11.1 | D347E | site-directed mutagenesis, inactive mutant | Solanum lycopersicum |
3.4.11.1 | D347R | site-directed mutagenesis, reduced activity | Solanum lycopersicum |
3.4.11.1 | E429D | site-directed mutagenesis, 95% activity compared to wild-type enzyme | Solanum lycopersicum |
3.4.11.1 | E429X | site-directed mutagenesis, inactive mutants, overview | Solanum lycopersicum |
3.4.11.1 | K354X | site-directed mutagenesis, inactive mutants, overview | Solanum lycopersicum |
3.4.11.1 | R431K | site-directed mutagenesis, 4fold reduced activity | Solanum lycopersicum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | amastatin | inhibition of LAP-A1 | Solanum lycopersicum | |
3.4.11.1 | bestatin | transition-state analogue, complete inhibition | Phaseolus vulgaris | |
3.4.11.1 | bestatin | inhibition of LAP-A1 | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.1 | 0.5 | - |
L-Leu-4-nitroanilide | - |
Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.1 | Mg2+ | activates | Hordeum vulgare | |
3.4.11.1 | Mg2+ | activates | Spinacia oleracea | |
3.4.11.1 | Mg2+ | activates | Brassica sp. | |
3.4.11.1 | Mg2+ | activates, LAP-A1 | Solanum lycopersicum | |
3.4.11.1 | Mn2+ | activates | Hordeum vulgare | |
3.4.11.1 | Mn2+ | activates | Spinacia oleracea | |
3.4.11.1 | Mn2+ | activates | Brassica sp. | |
3.4.11.1 | Mn2+ | activates, LAP-A1 | Solanum lycopersicum | |
3.4.11.1 | additional information | metallopeptidase | Phaseolus vulgaris | |
3.4.11.1 | additional information | metallopeptidase | Hordeum vulgare | |
3.4.11.1 | additional information | metallopeptidase | Spinacia oleracea | |
3.4.11.1 | additional information | metallopeptidase | Solanum tuberosum | |
3.4.11.1 | additional information | metallopeptidase | Arabidopsis thaliana | |
3.4.11.1 | additional information | metallopeptidase | Brassica sp. | |
3.4.11.1 | Zn2+ | metallopeptidase, bound by residues Asp347 and Glu429 | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 55000 | - |
6 * 55000, wound-induced LAP-A | Solanum lycopersicum |
3.4.11.1 | 55000 | - |
x * 55000, LAP-A | Solanum tuberosum |
3.4.11.1 | 58000 | - |
x * 58000 + x * 66000 | Phaseolus vulgaris |
3.4.11.1 | 66000 | - |
x * 58000 + x * 66000 | Phaseolus vulgaris |
3.4.11.1 | 320000 | 360000 | - |
Phaseolus vulgaris |
3.4.11.1 | 320000 | 360000 | - |
Hordeum vulgare |
3.4.11.1 | 320000 | 360000 | - |
Spinacia oleracea |
3.4.11.1 | 320000 | 360000 | - |
Solanum tuberosum |
3.4.11.1 | 320000 | 360000 | - |
Arabidopsis thaliana |
3.4.11.1 | 320000 | 360000 | - |
Solanum lycopersicum |
3.4.11.1 | 320000 | 360000 | - |
Brassica sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.1 | Arabidopsis thaliana | - |
- |
- |
3.4.11.1 | Brassica sp. | - |
cabbage | - |
3.4.11.1 | Hordeum vulgare | - |
- |
- |
3.4.11.1 | Phaseolus vulgaris | - |
kidney bean | - |
3.4.11.1 | Solanum lycopersicum | - |
LAP-A, encoded by genes LAP-A1 and LAP-A2 | - |
3.4.11.1 | Solanum tuberosum | - |
- |
- |
3.4.11.1 | Spinacia oleracea | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.11.1 | proteolytic modification | the pre-protein is processed to its mature form | Solanum tuberosum |
3.4.11.1 | proteolytic modification | the pre-protein is processed to its mature form | Solanum lycopersicum |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.1 | native enzyme, and recombinant enzyme from Escherichia coli | Phaseolus vulgaris |
3.4.11.1 | native enzyme, and recombinant enzyme from Escherichia coli | Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.11.1 | leaf | - |
Solanum lycopersicum | - |
3.4.11.1 | seed | germinated | Hordeum vulgare | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.1 | L-Ala-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Ala + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Arg-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Arg + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Arg-4-nitroanilide + H2O | pre-LAP | Solanum tuberosum | L-Arg + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Ile-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Ile + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Leu-4-nitroanilide + H2O | - |
Arabidopsis thaliana | L-Leu + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Leu-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Leu + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Leu-4-nitroanilide + H2O | pre-LAP | Solanum tuberosum | L-Leu + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Met-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Met + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Met-4-nitroanilide + H2O | pre-LAP | Solanum tuberosum | L-Met + 4-nitroaniline | - |
? | |
3.4.11.1 | L-Val-4-nitroanilide + H2O | - |
Solanum lycopersicum | L-Val + 4-nitroaniline | - |
? | |
3.4.11.1 | Leu-systemin + H2O | LAP-A1, only in vitro | Solanum lycopersicum | Leu + systemin | - |
? | |
3.4.11.1 | additional information | no activity towards substrates with Asp or Glu at position P1, Arg431 and Lys354 of LAP-A1 are involved in catalysis | Solanum lycopersicum | ? | - |
? | |
3.4.11.1 | additional information | the enzyme hydrolyses with Leu or Met in the P1 position | Hordeum vulgare | ? | - |
? | |
3.4.11.1 | additional information | the enzyme hydrolyses with Leu or Met in the P1 position, the residues at P1', P2'a nd P3' positions influence the activity | Phaseolus vulgaris | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.1 | ? | x * 55000, LAP-A | Solanum tuberosum |
3.4.11.1 | hexamer | 6 * 55000, wound-induced LAP-A | Solanum lycopersicum |
3.4.11.1 | hexamer | x * 58000 + x * 66000 | Phaseolus vulgaris |
3.4.11.1 | multimer | - |
Hordeum vulgare |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.1 | LAP | - |
Phaseolus vulgaris |
3.4.11.1 | LAP | - |
Hordeum vulgare |
3.4.11.1 | LAP-A | - |
Solanum tuberosum |
3.4.11.1 | LAP-A | - |
Solanum lycopersicum |
3.4.11.1 | leucyl aminopeptidase (plant) | - |
Phaseolus vulgaris |
3.4.11.1 | leucyl aminopeptidase (plant) | - |
Hordeum vulgare |
3.4.11.1 | leucyl aminopeptidase (plant) | - |
Spinacia oleracea |
3.4.11.1 | leucyl aminopeptidase (plant) | - |
Solanum tuberosum |
3.4.11.1 | leucyl aminopeptidase (plant) | - |
Arabidopsis thaliana |
3.4.11.1 | leucyl aminopeptidase (plant) | - |
Solanum lycopersicum |
3.4.11.1 | leucyl aminopeptidase (plant) | - |
Brassica sp. |
3.4.11.1 | leucylpeptidase | - |
Hordeum vulgare |
3.4.11.1 | leucylpeptidase | - |
Spinacia oleracea |
3.4.11.1 | leucylpeptidase | - |
Brassica sp. |
3.4.11.1 | More | the enzyme belongs to peptidase family M17 | Phaseolus vulgaris |
3.4.11.1 | More | the enzyme belongs to peptidase family M17 | Hordeum vulgare |
3.4.11.1 | More | the enzyme belongs to peptidase family M17 | Spinacia oleracea |
3.4.11.1 | More | the enzyme belongs to peptidase family M17 | Solanum tuberosum |
3.4.11.1 | More | the enzyme belongs to peptidase family M17 | Arabidopsis thaliana |
3.4.11.1 | More | the enzyme belongs to peptidase family M17 | Solanum lycopersicum |
3.4.11.1 | More | the enzyme belongs to peptidase family M17 | Brassica sp. |
3.4.11.1 | peptidase II | - |
Hordeum vulgare |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | additional information | - |
the enzyme is thermostable | Phaseolus vulgaris |
3.4.11.1 | additional information | - |
the enzyme is thermostable | Hordeum vulgare |
3.4.11.1 | additional information | - |
the enzyme is thermostable | Spinacia oleracea |
3.4.11.1 | additional information | - |
the enzyme is thermostable | Solanum tuberosum |
3.4.11.1 | additional information | - |
the enzyme is thermostable | Arabidopsis thaliana |
3.4.11.1 | additional information | - |
the enzyme is thermostable | Solanum lycopersicum |
3.4.11.1 | additional information | - |
the enzyme is thermostable | Brassica sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.1 | 8.5 | 9.5 | - |
Phaseolus vulgaris |
3.4.11.1 | 8.5 | 9.5 | - |
Hordeum vulgare |
3.4.11.1 | 8.5 | 9.5 | - |
Spinacia oleracea |
3.4.11.1 | 8.5 | 9.5 | - |
Solanum tuberosum |
3.4.11.1 | 8.5 | 9.5 | - |
Arabidopsis thaliana |
3.4.11.1 | 8.5 | 9.5 | - |
Solanum lycopersicum |
3.4.11.1 | 8.5 | 9.5 | - |
Brassica sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.1 | 0.0000015 | - |
bestatin | - |
Phaseolus vulgaris |