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Literature summary extracted from

  • Walling, L.L.
    Leucyl aminopeptidase (plant) (2004), Handbook of Proteolytic Enzymes (2nd Edition), 1, 901-905.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.1 additional information wounding induces LAP-A Solanum tuberosum
3.4.11.1 additional information wounding induces LAP-A, which is also induced by salt- and water-stress, and pathogen attack Solanum lycopersicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 DNA sequence determination, overexpression of a LAP fusion protein in Escherichia coli Arabidopsis thaliana
3.4.11.1 DNA sequence determination, overexpression of mature LAP-A, His6-tagged LAP-A and pre-LAP-A in Escherichia coli Solanum lycopersicum
3.4.11.1 DNA sequence determination, overexpression of pre-LAP protein in Escherichia coli Solanum tuberosum
3.4.11.1 overexpression in Escherichia coli Phaseolus vulgaris
3.4.11.1 overexpression in Escherichia coli Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.1 D347E site-directed mutagenesis, inactive mutant Solanum lycopersicum
3.4.11.1 D347R site-directed mutagenesis, reduced activity Solanum lycopersicum
3.4.11.1 E429D site-directed mutagenesis, 95% activity compared to wild-type enzyme Solanum lycopersicum
3.4.11.1 E429X site-directed mutagenesis, inactive mutants, overview Solanum lycopersicum
3.4.11.1 K354X site-directed mutagenesis, inactive mutants, overview Solanum lycopersicum
3.4.11.1 R431K site-directed mutagenesis, 4fold reduced activity Solanum lycopersicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 amastatin inhibition of LAP-A1 Solanum lycopersicum
3.4.11.1 bestatin transition-state analogue, complete inhibition Phaseolus vulgaris
3.4.11.1 bestatin inhibition of LAP-A1 Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.5
-
L-Leu-4-nitroanilide
-
Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ activates Hordeum vulgare
3.4.11.1 Mg2+ activates Spinacia oleracea
3.4.11.1 Mg2+ activates Brassica sp.
3.4.11.1 Mg2+ activates, LAP-A1 Solanum lycopersicum
3.4.11.1 Mn2+ activates Hordeum vulgare
3.4.11.1 Mn2+ activates Spinacia oleracea
3.4.11.1 Mn2+ activates Brassica sp.
3.4.11.1 Mn2+ activates, LAP-A1 Solanum lycopersicum
3.4.11.1 additional information metallopeptidase Phaseolus vulgaris
3.4.11.1 additional information metallopeptidase Hordeum vulgare
3.4.11.1 additional information metallopeptidase Spinacia oleracea
3.4.11.1 additional information metallopeptidase Solanum tuberosum
3.4.11.1 additional information metallopeptidase Arabidopsis thaliana
3.4.11.1 additional information metallopeptidase Brassica sp.
3.4.11.1 Zn2+ metallopeptidase, bound by residues Asp347 and Glu429 Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 55000
-
6 * 55000, wound-induced LAP-A Solanum lycopersicum
3.4.11.1 55000
-
x * 55000, LAP-A Solanum tuberosum
3.4.11.1 58000
-
x * 58000 + x * 66000 Phaseolus vulgaris
3.4.11.1 66000
-
x * 58000 + x * 66000 Phaseolus vulgaris
3.4.11.1 320000 360000
-
Phaseolus vulgaris
3.4.11.1 320000 360000
-
Hordeum vulgare
3.4.11.1 320000 360000
-
Spinacia oleracea
3.4.11.1 320000 360000
-
Solanum tuberosum
3.4.11.1 320000 360000
-
Arabidopsis thaliana
3.4.11.1 320000 360000
-
Solanum lycopersicum
3.4.11.1 320000 360000
-
Brassica sp.

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Arabidopsis thaliana
-
-
-
3.4.11.1 Brassica sp.
-
cabbage
-
3.4.11.1 Hordeum vulgare
-
-
-
3.4.11.1 Phaseolus vulgaris
-
kidney bean
-
3.4.11.1 Solanum lycopersicum
-
LAP-A, encoded by genes LAP-A1 and LAP-A2
-
3.4.11.1 Solanum tuberosum
-
-
-
3.4.11.1 Spinacia oleracea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.1 proteolytic modification the pre-protein is processed to its mature form Solanum tuberosum
3.4.11.1 proteolytic modification the pre-protein is processed to its mature form Solanum lycopersicum

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 native enzyme, and recombinant enzyme from Escherichia coli Phaseolus vulgaris
3.4.11.1 native enzyme, and recombinant enzyme from Escherichia coli Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 leaf
-
Solanum lycopersicum
-
3.4.11.1 seed germinated Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Ala-4-nitroanilide + H2O
-
Solanum lycopersicum L-Ala + 4-nitroaniline
-
?
3.4.11.1 L-Arg-4-nitroanilide + H2O
-
Solanum lycopersicum L-Arg + 4-nitroaniline
-
?
3.4.11.1 L-Arg-4-nitroanilide + H2O pre-LAP Solanum tuberosum L-Arg + 4-nitroaniline
-
?
3.4.11.1 L-Ile-4-nitroanilide + H2O
-
Solanum lycopersicum L-Ile + 4-nitroaniline
-
?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
Arabidopsis thaliana L-Leu + 4-nitroaniline
-
?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
Solanum lycopersicum L-Leu + 4-nitroaniline
-
?
3.4.11.1 L-Leu-4-nitroanilide + H2O pre-LAP Solanum tuberosum L-Leu + 4-nitroaniline
-
?
3.4.11.1 L-Met-4-nitroanilide + H2O
-
Solanum lycopersicum L-Met + 4-nitroaniline
-
?
3.4.11.1 L-Met-4-nitroanilide + H2O pre-LAP Solanum tuberosum L-Met + 4-nitroaniline
-
?
3.4.11.1 L-Val-4-nitroanilide + H2O
-
Solanum lycopersicum L-Val + 4-nitroaniline
-
?
3.4.11.1 Leu-systemin + H2O LAP-A1, only in vitro Solanum lycopersicum Leu + systemin
-
?
3.4.11.1 additional information no activity towards substrates with Asp or Glu at position P1, Arg431 and Lys354 of LAP-A1 are involved in catalysis Solanum lycopersicum ?
-
?
3.4.11.1 additional information the enzyme hydrolyses with Leu or Met in the P1 position Hordeum vulgare ?
-
?
3.4.11.1 additional information the enzyme hydrolyses with Leu or Met in the P1 position, the residues at P1', P2'a nd P3' positions influence the activity Phaseolus vulgaris ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 55000, LAP-A Solanum tuberosum
3.4.11.1 hexamer 6 * 55000, wound-induced LAP-A Solanum lycopersicum
3.4.11.1 hexamer x * 58000 + x * 66000 Phaseolus vulgaris
3.4.11.1 multimer
-
Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP
-
Phaseolus vulgaris
3.4.11.1 LAP
-
Hordeum vulgare
3.4.11.1 LAP-A
-
Solanum tuberosum
3.4.11.1 LAP-A
-
Solanum lycopersicum
3.4.11.1 leucyl aminopeptidase (plant)
-
Phaseolus vulgaris
3.4.11.1 leucyl aminopeptidase (plant)
-
Hordeum vulgare
3.4.11.1 leucyl aminopeptidase (plant)
-
Spinacia oleracea
3.4.11.1 leucyl aminopeptidase (plant)
-
Solanum tuberosum
3.4.11.1 leucyl aminopeptidase (plant)
-
Arabidopsis thaliana
3.4.11.1 leucyl aminopeptidase (plant)
-
Solanum lycopersicum
3.4.11.1 leucyl aminopeptidase (plant)
-
Brassica sp.
3.4.11.1 leucylpeptidase
-
Hordeum vulgare
3.4.11.1 leucylpeptidase
-
Spinacia oleracea
3.4.11.1 leucylpeptidase
-
Brassica sp.
3.4.11.1 More the enzyme belongs to peptidase family M17 Phaseolus vulgaris
3.4.11.1 More the enzyme belongs to peptidase family M17 Hordeum vulgare
3.4.11.1 More the enzyme belongs to peptidase family M17 Spinacia oleracea
3.4.11.1 More the enzyme belongs to peptidase family M17 Solanum tuberosum
3.4.11.1 More the enzyme belongs to peptidase family M17 Arabidopsis thaliana
3.4.11.1 More the enzyme belongs to peptidase family M17 Solanum lycopersicum
3.4.11.1 More the enzyme belongs to peptidase family M17 Brassica sp.
3.4.11.1 peptidase II
-
Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 additional information
-
the enzyme is thermostable Phaseolus vulgaris
3.4.11.1 additional information
-
the enzyme is thermostable Hordeum vulgare
3.4.11.1 additional information
-
the enzyme is thermostable Spinacia oleracea
3.4.11.1 additional information
-
the enzyme is thermostable Solanum tuberosum
3.4.11.1 additional information
-
the enzyme is thermostable Arabidopsis thaliana
3.4.11.1 additional information
-
the enzyme is thermostable Solanum lycopersicum
3.4.11.1 additional information
-
the enzyme is thermostable Brassica sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8.5 9.5
-
Phaseolus vulgaris
3.4.11.1 8.5 9.5
-
Hordeum vulgare
3.4.11.1 8.5 9.5
-
Spinacia oleracea
3.4.11.1 8.5 9.5
-
Solanum tuberosum
3.4.11.1 8.5 9.5
-
Arabidopsis thaliana
3.4.11.1 8.5 9.5
-
Solanum lycopersicum
3.4.11.1 8.5 9.5
-
Brassica sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.1 0.0000015
-
bestatin
-
Phaseolus vulgaris