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Literature summary extracted from

  • Liu, C.; Huang, L.; Chang, C.; Sung, H.
    Purification and characterization of soluble invertases from suspension-cultured bamboo (Bambusa edulis) cells (2006), Food Chem., 96, 621-631.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.26 bovine serum albumin activates isozyme IT II best at 0.2 mg/ml, and isozyme IT I slightly at 100 mg/ml Phyllostachys edulis
3.2.1.26 concanavalin A activates isozyme IT II best at 0.9 mg/ml Phyllostachys edulis
3.2.1.26 DTNB 0.5 mM, 11% activation of isozyme IT II, 24% inhibition of isozyme IT I Phyllostachys edulis
3.2.1.26 urease activates isozyme IT II best at 0.1 mg/ml Phyllostachys edulis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.26 4-chloromercuribenzoate 0.5 mM, 62% inhibition of isozyme IT I, 86% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 Ag+ 1 mM, 56% inhibition of isozyme IT I, 81% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 aniline 5 mM, 36% inhibition of isozyme IT I, 17% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 Ca2+ 5 mM, 35% inhibition of isozyme IT I, 84% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 Cu2+ 1 mM, 53% inhibition of isozyme IT I, 85% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 D-fructose product inhibition, 2.5 mM, 62% inhibition of isozyme IT I, 70% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 D-glucose product inhibition, 2.5 mM, 58% inhibition of isozyme IT I, 72% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 DTNB 0.5 mM, 24% inhibition of isozyme IT I, 11% activation of isozyme IT II Phyllostachys edulis
3.2.1.26 Hg2+ 1 mM, 93% inhibition of isozyme IT I, 95% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 iodoacetamide 5 mM, about 20% inhibition of isozyme IT I, 44% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 Mg2+ 1 mM, 11% inhibition of isozyme IT I, 14% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 PMSF 0.5 mM, 20% inhibition of isozyme IT I, 60% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 pyridoxine 5 mM, 48% inhibition of isozyme IT I, 75% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 sucrose substrate inhibition, 400 mM: 10% inhibition of isozyme IT I, 20% inhibition of isozyme IT II, 1 M: 10% inhibition of isozyme IT I, 62% inhibition of isozyme IT II Phyllostachys edulis
3.2.1.26 Zn2+ 1 mM, no inhibition of isozyme IT I, 86% inhibition of isozyme IT II Phyllostachys edulis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 3.7
-
 sucrose pH 5.0, 37°C, isozyme IT II Phyllostachys edulis
3.2.1.26 10.9
-
 sucrose pH 7.0, 37°C, isozyme IT I Phyllostachys edulis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 soluble soluble alkaline and acid invertase isozymes, i.e. IT I and IT II Phyllostachys edulis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 60000
-
 4 * 60000, isozyme IT I, SDS-PAGE Phyllostachys edulis
3.2.1.26 67000
-
 1 * 67000, isozyme IT II, SDS-PAGE Phyllostachys edulis
3.2.1.26 68000
-
 isozyme IT II, gel filtration Phyllostachys edulis
3.2.1.26 240000
-
 isozyme IT I, gel filtration Phyllostachys edulis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Phyllostachys edulis
-
 isozymes alkaline and acid invertases, i.e. IT I and IT II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 native soluble alkaline isozyme IT I 144fold from cell suspension culture to homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, and native soluble acid invertase isozyme IT II 130fold from cell suspension culture to homogeneity by ammonium sulfate fractionation, anion exchange and cation exchange chromatography, ConA affinity chromatography, and anion exchange chromatography Phyllostachys edulis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 cell suspension culture
-
 Phyllostachys edulis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.26 26
-
 purified isozyme IT II Phyllostachys edulis
3.2.1.26 28.8
-
 purified isozyme IT I Phyllostachys edulis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 additional information both isozymes IT I and IT II show no activity with maltose Phyllostachys edulis ?
-
 ?
3.2.1.26 raffinose + H2O isozymes IT I and IT II Phyllostachys edulis alpha-D-galactosyl-1,6-alpha-D-glucose + D-fructose
-
 ?
3.2.1.26 sucrose + H2O isozymes IT I and IT II Phyllostachys edulis D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.26 monomer 1 * 67000, isozyme IT II, SDS-PAGE Phyllostachys edulis
3.2.1.26 tetramer 4 * 60000, isozyme IT I, SDS-PAGE Phyllostachys edulis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 acid invertase
-
 Phyllostachys edulis
3.2.1.26 alkaline invertase
-
 Phyllostachys edulis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.26 30 40 both isozyme IT I and isozyme IT II Phyllostachys edulis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.26 50
-
 10 min, both isozymes IT I and IT II retain over 70% activity Phyllostachys edulis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.26 4
-
 acid invertase isozyme IT II Phyllostachys edulis
3.2.1.26 7 7.5 alkaline invertase isozyme IT I Phyllostachys edulis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.26 Phyllostachys edulis alkaline invertase isozyme IT I, isoelectric focusing
-
 4.8
3.2.1.26 Phyllostachys edulis acid invertase isozyme IT II, isoelectric focusing
-
 7.4