Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tsao, C.; Hsu, Y.; Chao, L.; Jiang, S.
    Purification and characterization of three amylases from viscera of hard clam Meretrix lusoria (2004), Fish. Sci., 70, 174-182.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Al3+ complete inhibition of isozyme AI-1, nearly complete inhibition of isozyme AI-2, moderate inhibition of isozyme AII, at 5 mM Meretrix lusoria
3.2.1.1 Ba2+ slight inhibition of isozymes AI-1 and AI-2, and AII Meretrix lusoria
3.2.1.1 Cd2+ complete inhibition of isozyme AII, high inhibition of isozymes AI-1 and moderate of AI-2, at 5 mM Meretrix lusoria
3.2.1.1 Co2+ slight activation of isozyme AI-2, slight inhibition of isozymes AI-1 and AII Meretrix lusoria
3.2.1.1 Cu2+ nearly complete inhibition of isozymes AI-1 and AI-2, and AII at 5 mM Meretrix lusoria
3.2.1.1 DTNB inhibition of isozymes AI-1 and AI-2, and AII, at 5 mM Meretrix lusoria
3.2.1.1 EDTA strong inhibition of isozymes AI-1 and AI-2, and AII, at 5 mM Meretrix lusoria
3.2.1.1 Fe3+ complete inhibition of isozymes AI-1 and AI-2, and AII at 5 mM Meretrix lusoria
3.2.1.1 Hg2+ complete inhibition of isozymes AI-1 and AI-2, and AII at 5 mM Meretrix lusoria
3.2.1.1 iodoacetic acid slight inhibition of isozymes AI-1 and AI-2, and AII, at 5 mM Meretrix lusoria
3.2.1.1 K+ slight activation of isozymes AI-1 and AII, slight inhibition of isozyme AI-2 Meretrix lusoria
3.2.1.1 Li+ slight activation of isozymes AI-1 and AI-2, slight inhibition of isozyme AII Meretrix lusoria
3.2.1.1 NEM inhibition of isozymes AI-1 and AI-2, and AII, at 5 mM Meretrix lusoria
3.2.1.1 Ni2+ high inhibition of isozymes AI-1 and AI-2, and AII Meretrix lusoria
3.2.1.1 PMSF inhibition of isozymes AI-1 and AI-2, and AII, at 5 mM Meretrix lusoria
3.2.1.1 Sr2+ slight inhibition of isozymes AI-1 and AI-2, and AII Meretrix lusoria
3.2.1.1 Urea inhibition of isozymes AI-1 and AI-2, and AII, at 5 mM Meretrix lusoria
3.2.1.1 Zn2+ complete inhibition of isozyme AI-1, high inhibition of isozymes AII and AI-2, at 5 mM Meretrix lusoria
3.2.1.3 Al3+
-
 Meretrix lusoria
3.2.1.3 Ba2+ slight inhibition Meretrix lusoria
3.2.1.3 Cd2+ complete inhibition Meretrix lusoria
3.2.1.3 Co2+
-
 Meretrix lusoria
3.2.1.3 Cu2+ strong inhibition Meretrix lusoria
3.2.1.3 EDTA strong inhibition Meretrix lusoria
3.2.1.3 Fe3+ complete inhibition Meretrix lusoria
3.2.1.3 Hg2+ complete inhibition Meretrix lusoria
3.2.1.3 additional information iodoacetamide and urea are poor inhibitors Meretrix lusoria
3.2.1.3 NEM slight inhibition Meretrix lusoria
3.2.1.3 Ni2+ strong inhibition Meretrix lusoria
3.2.1.3 PMSF slight inhibition Meretrix lusoria
3.2.1.3 Sr2+ slight inhibition Meretrix lusoria
3.2.1.3 Zn2+ strong inhibition Meretrix lusoria

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Co2+ slight activation of isozyme AI-2, slight inhibition of isozymes AI-1 and AII Meretrix lusoria
3.2.1.1 K+ slight activation of isozymes AI-1 and AII, slight inhibition of isozyme AI-2 Meretrix lusoria
3.2.1.1 Li+ slight activation of isozymes AI-1 and AI-2, slight inhibition of isozyme AII Meretrix lusoria
3.2.1.1 Mg2+ slight activation of isozymes AI-1 and AI-2, and AII Meretrix lusoria
3.2.1.1 Mn2+ activation of isozymes AI-1 and AI-2, and AII Meretrix lusoria
3.2.1.1 additional information no effect on isozymes by Ca2+ Meretrix lusoria
3.2.1.1 Na+ activation of isozymes AI-1 and AI-2, and AII Meretrix lusoria
3.2.1.3 Mn2+ activates Meretrix lusoria
3.2.1.3 additional information no requirement for Ca2+, the enzyme is not affected by K+, and Mg2+ Meretrix lusoria
3.2.1.3 Na+ activates Meretrix lusoria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 49600
-
 x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE Meretrix lusoria
3.2.1.1 56700
-
 x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE Meretrix lusoria
3.2.1.1 100000
-
 x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE Meretrix lusoria
3.2.1.3 100000
-
 x * 100000, SDS-PAGE Meretrix lusoria

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Meretrix lusoria
-
 hard clam, three isozymes AI-1 and AI-2, and AII
-
3.2.1.3 Meretrix lusoria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 native three isozymes AI-1 and AI-2, and AII 1076fold, 1813fold, and 18fold, respectively, from viscera, to homogeneity by ammonium sulfate fractionation, and two alternating, sequential steps of both gel filtration and ion exchange chromatography Meretrix lusoria
3.2.1.3 native enzyme 18fold from viscus, by ammonium sulfate fractionation, alternating gel filtration and ion exchange chromatography steps, to homogeneity Meretrix lusoria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 viscus
-
 Meretrix lusoria
-
3.2.1.3 viscus
-
 Meretrix lusoria
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 46.8
-
 purified enzyme Meretrix lusoria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O isozymes AI-1 and AI-2, and AII, good substrate Meretrix lusoria ?
-
 ?
3.2.1.1 amylose + H2O isozymes AI-1 and AI-2, and AII, moderate activity Meretrix lusoria ?
-
 ?
3.2.1.1 gamma-cyclodextrin + H2O lower activity with isozyme AI-2, no activity with isozymes AI-1 and AII Meretrix lusoria ?
-
 ?
3.2.1.1 glycogen + H2O isozymes AI-1 and AI-2, and AII, good substrate Meretrix lusoria ?
-
 ?
3.2.1.1 laminarin + H2O lower activity with isozyme AI-1, low activity with isozymes AI-2 and AII Meretrix lusoria ?
-
 ?
3.2.1.1 maltopentaose + H2O isozymes AI-1 and AI-2, and AII, low activity Meretrix lusoria ?
-
 ?
3.2.1.1 maltotetraose + H2O isozymes AI-1 and AI-2, and AII, low activity Meretrix lusoria ?
-
 ?
3.2.1.1 maltotriose + H2O lower activity with isozyme AII, no activity with isozymes AI-1 and AI-2 Meretrix lusoria ?
-
 ?
3.2.1.1 additional information isozyme substrate specificity, no activity of all isozymes with pullulan, alpha-cyclodextrin, and beta-cyclodextrin, overview Meretrix lusoria ?
-
 ?
3.2.1.1 starch + H2O soluble starch, best substrate for isozymes AI-1 and AI-2, and AII Meretrix lusoria ?
-
 ?
3.2.1.3 amylopectin + H2O exoglycosidic hydrolysis of terminal glucose, best substrate Meretrix lusoria amylopectin + beta-D-glucose
-
 ?
3.2.1.3 amylose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria amylose + beta-D-glucose
-
 ?
3.2.1.3 glycogen + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria glycogen + beta-D-glucose
-
 ?
3.2.1.3 maltopentaose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria maltotetraose + beta-D-glucose
-
 ?
3.2.1.3 maltotetraose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria maltotriose + beta-D-glucose
-
 ?
3.2.1.3 maltotriose + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria maltose + beta-D-glucose
-
 ?
3.2.1.3 additional information no activity with cyclodextrins and laminarin Meretrix lusoria ?
-
 ?
3.2.1.3 pullulan + H2O exoglycosidic hydrolysis of terminal glucose Meretrix lusoria pullulan + beta-D-glucose
-
 ?
3.2.1.3 starch + H2O exoglycosidic hydrolysis of terminal glucose, high activity Meretrix lusoria starch + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 49600, isozyme AI-1, SDS-PAGE, x * 56700, isozyme AI-2, SDS-PAGE, x * 100000, isozyme AII, SDS-PAGE Meretrix lusoria
3.2.1.3 ? x * 100000, SDS-PAGE Meretrix lusoria

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 amylase AI
-
 Meretrix lusoria
3.2.1.1 amylase AII
-
 Meretrix lusoria
3.2.1.3 exo-amylase
-
 Meretrix lusoria
3.2.1.3 gamma-amylase
-
 Meretrix lusoria

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 35 45 isozyme AI-1 Meretrix lusoria
3.2.1.1 50
-
 isozymes AI-2 and AII Meretrix lusoria
3.2.1.3 50
-
-
 Meretrix lusoria

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 25 50 activity in this range depends on the buffer system used, overview Meretrix lusoria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 40
-
 30 min, stable up to Meretrix lusoria
3.2.1.1 50
-
 30 min, inactivation Meretrix lusoria
3.2.1.3 40
-
 stable up to Meretrix lusoria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6.5
-
 about, isozyme AI-1 Meretrix lusoria
3.2.1.1 7
-
 isozyme AII Meretrix lusoria
3.2.1.1 7.5
-
 about, isozyme AI-2 Meretrix lusoria
3.2.1.3 7.5
-
-
 Meretrix lusoria

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5 8 12 h, isozymes AI-1 and AI-2, and AII, most stable at pH 7.0, about 50% of maximal activity at pH 3.5-4.5 and at pH 8.0-8.5, dependent on the isozyme, overview Meretrix lusoria

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.1 Meretrix lusoria isozyme AI-2 10 4
3.2.1.1 Meretrix lusoria isozyme AI-1 9 4
3.2.1.1 Meretrix lusoria isozyme AII 8 4