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Literature summary extracted from

  • Pioselli, B.; Bettati, S.; Mozzarelli, A.
    Confinement and crowding effects on tryptophan synthase alpha2beta2 complex (2005), FEBS Lett., 579, 2197-2202.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 0.094
-
 indole 37°C, beta-replacement, enzyme in solution Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 0.15
-
 indole 37°C, beta-replacement, enzyme encapsulated in tetramethylorthosilicate-derived wet silica gels Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 0.98
-
 L-serine 37°C, beta-replacement, enzyme in solution Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 1.16
-
 L-serine 37°C, beta-elimination, enzyme in solution Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 1.17
-
 L-serine 37°C, beta-replacement, enzyme encapsulated in tetramethylorthosilicate-derived wet silica gels Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 1.41
-
 L-serine 37°C, beta-elimination, enzyme encapsulated in tetramethylorthosilicate-derived wet silica gels Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Salmonella enterica subsp. enterica serovar Typhimurium
-
 recombinant
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 L-serine + indole
-
 Salmonella enterica subsp. enterica serovar Typhimurium tryptophan + H2O
-
 ?
4.2.1.20 additional information in the case of silica gel-encapsulated enzyme, the altered equilibrium distribution of tertiary conformations can not be totally ascribed to caging and viscosity effects, but might have a strong contribution from protein matrix intercation Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.20 0.15
-
 L-serine 37°C, beta-elimination, enzyme encapsulated in tetramethylorthosilicate-derived wet silica gels Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 0.16
-
 L-serine 37°C, beta-replacement, enzyme encapsulated in tetramethylorthosilicate-derived wet silica gels Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 0.26
-
 L-serine 37°C, beta-elimination, enzyme in solution Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 3.3
-
 indole 37°C, beta-replacement, enzyme encapsulated in tetramethylorthosilicate-derived wet silica gels Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 8.2
-
 L-serine 37°C, beta-replacement, enzyme in solution Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.20 10.1
-
 indole 37°C, beta-replacement, enzyme in solution Salmonella enterica subsp. enterica serovar Typhimurium