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Literature summary extracted from

  • Matsui, K.; Fukutomi, S.; Ishii, M.; Kajiwara, T.
    A tomato lipase homologous to DAD1 (LeLID1) is induced in post-germinative growing stage and encodes a triacylglycerol lipase (2004), FEBS Lett., 569, 195-200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli, native enzyme partially by subcellular fractionation Solanum lycopersicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 additional information no enzyme in the oil body fraction Solanum lycopersicum
-
-
3.1.1.3 soluble
-
 Solanum lycopersicum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 47500
-
 x * 47500, recombinant His-tagged enzyme, SDS-PAGE Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 additional information Solanum lycopersicum the enzyme probably is involved in fat mobilization during seed germination ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Solanum lycopersicum
-
 cv. Momotarou
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant His-tagged enzyme from Escherichia coli by Co2+ affinity chromatography Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 cotyledon high expression level Solanum lycopersicum
-
3.1.1.3 flower low expression level Solanum lycopersicum
-
3.1.1.3 fruit low expression level Solanum lycopersicum
-
3.1.1.3 hypocotyl
-
 Solanum lycopersicum
-
3.1.1.3 additional information no enzyme expression in root Solanum lycopersicum
-
3.1.1.3 seed high increase in enzyme expression during germination Solanum lycopersicum
-
3.1.1.3 seedling maximum activity 4 days after germination Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information the enzyme probably is involved in fat mobilization during seed germination Solanum lycopersicum ?
-
 ?
3.1.1.3 additional information substrate specificity, the enzyme catalyzes hydrolysis of triacylglycerides with long fatty acid chains, low activity with tripalmitin and tristearin, no activity with tributyrin and triacetate, phosphatidylcholine and monogalactosyldiacylglycerol are poor substrates Solanum lycopersicum ?
-
 ?
3.1.1.3 tricaprylin + H2O
-
 Solanum lycopersicum dicaprylin + caprylate
-
 ?
3.1.1.3 triolein + H2O
-
 Solanum lycopersicum diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O low activity Solanum lycopersicum dipalmitin + palmitate
-
 ?
3.1.1.3 tristearin + H2O low activity Solanum lycopersicum distearin + stearate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 47500, recombinant His-tagged enzyme, SDS-PAGE Solanum lycopersicum

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 L1D1
-
 Solanum lycopersicum
3.1.1.3 lipase homologous to DAD1
-
 Solanum lycopersicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 25
-
 assay at Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 recombinant enzyme Solanum lycopersicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 additional information
-
 activity profile of the recombinant His-tagged enzyme Solanum lycopersicum