Literature summary extracted from

Suresh, C.; Rusd, A.A.; Kitaoka, M.; Hayashi, K.
Evidence that the putative alpha-glucosidase of Thermotoga maritima MSB8 is a pNP alpha-D-glucuronopyranoside hydrolyzing alpha-glucuronidase (2002), FEBS Lett., 517, 159-162.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.139	expression in Escherichia coli	Thermotoga maritima

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.2.1.139	DTT	50 mM, required for maximal activity	Thermotoga maritima

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.139	1	-	p-nitrophenyl-alpha-D-glucuronopyranoside	-	Thermotoga maritima

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.2.1.139	Mn2+	slightly increases activity, 1 mM required for maximal activity	Thermotoga maritima

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.139	Thermotoga maritima	-	MSB8	-
3.2.1.139	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	-	MSB8	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.139	recombinant	Thermotoga maritima

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.139	p-nitrophenyl-alpha-D-glucuronopyranoside + H2O	-	Thermotoga maritima	p-nitrophenol + D-glucuronate	-	?
3.2.1.139	p-nitrophenyl-alpha-D-glucuronopyranoside + H2O	-	Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589	p-nitrophenol + D-glucuronate	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.2.1.139	60	-	-	Thermotoga maritima

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.2.1.139	0.0018	-	p-nitrophenyl-alpha-D-glucuronopyranoside	-	Thermotoga maritima

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.2.1.139	7.8	-	-	Thermotoga maritima

pH Stability

EC Number	pH Stability	pH Stability Maximum	Comment	Organism
3.2.1.139	5	11	room temperature, 30 min, stable	Thermotoga maritima
3.2.1.139	11.5	-	room temperature, 30 min, 50% loss of activity	Thermotoga maritima

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Release 2023.1 (January 2023)