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Literature summary extracted from

  • Takeda, Y.; Aono, R.; Doukyu, N.
    Purification, characterization, and molecular cloning of organic-solvent-tolerant cholesterol esterase from cyclohexane-tolerant Burkholderia cepacia strain ST-200 (2006), Extremophiles, 10, 269-177.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.13 2-propanol 10% v/v, 460% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
3.1.1.13 acetone 10% v/v, 570% of initial activity, 30% v/v, 53% of initial activity Burkholderia cepacia
3.1.1.13 DMF 10% v/v, 540% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
3.1.1.13 DMSO 10% v/v, 500% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
3.1.1.13 ethanol 10% v/v, 580% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
3.1.1.13 methanol 10% v/v, 680% of initial activity, 30% v/v, 58% of initial activity Burkholderia cepacia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.13 Ag+ 1 mM, 72% residual activity Burkholderia cepacia
3.1.1.13 Cu2+ 1 mM, 61% residual activity Burkholderia cepacia
3.1.1.13 Hg2+ 1 mM, 11% residual activity Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.13 37000
-
 x * 37000, SDS-PAGE Burkholderia cepacia

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.13 2-propanol 10% v/v, 460% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
3.1.1.13 Acetone 10% v/v, 570% of initial activity, 30% v/v, 53% of initial activity Burkholderia cepacia
3.1.1.13 dimethyl formamide 10% v/v, 540% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
3.1.1.13 dimethyl sulfoxide 10% v/v, 500% of initial activity, 30% v/v, 110% of initial activity Burkholderia cepacia
3.1.1.13 Ethanol 10% v/v, 580% of initial activity, 30% v/v, 98% of initial activity Burkholderia cepacia
3.1.1.13 Methanol 10% v/v, 680% of initial activity, 30% v/v, 58% of initial activity Burkholderia cepacia
3.1.1.13 additional information enzyme is highly stable in various water-miscible organic solvents Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.13 Burkholderia cepacia Q75NT4
-
-
3.1.1.13 Burkholderia cepacia ST-200 Q75NT4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.13 culture fluid
-
 Burkholderia cepacia
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.13 5.1
-
 pH 7.0, 37°C Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.13 cholesterol acetate + H2O 3% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + acetic acid
-
 ?
3.1.1.13 cholesterol acetate + H2O 3% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + acetic acid
-
 ?
3.1.1.13 cholesterol butyrate + H2O 18% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + butyric acid
-
 ?
3.1.1.13 cholesterol butyrate + H2O 18% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + butyric acid
-
 ?
3.1.1.13 cholesterol decanoate + H2O 34% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + decanoic acid
-
 ?
3.1.1.13 cholesterol decanoate + H2O 34% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + decanoic acid
-
 ?
3.1.1.13 cholesterol hexanoate + H2O 29% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + hexanoic acid
-
 ?
3.1.1.13 cholesterol linoleate + H2O
-
 Burkholderia cepacia cholesterol + linoleic acid
-
 ?
3.1.1.13 cholesterol oleate + H2O 53% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + oleic acid
-
 ?
3.1.1.13 cholesterol oleate + H2O 53% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 cholesterol + oleic acid
-
 ?
3.1.1.13 cholesterol palmitate + H2O 5% of the activity with cholesteryl linoleate Burkholderia cepacia cholesterol + palmitic acid
-
 ?
3.1.1.13 p-nitrophenyl acetate + H2O 5% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + acetate
-
 ?
3.1.1.13 p-nitrophenyl acetate + H2O 5% of the activity with cholesteryl linoleate Burkholderia cepacia ST-200 p-nitrophenol + acetate
-
 ?
3.1.1.13 p-nitrophenyl hexanoate + H2O 53% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + hexanoic acid
-
 ?
3.1.1.13 p-nitrophenyl laurate + H2O 92% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + lauric acid
-
 ?
3.1.1.13 p-nitrophenyl myristate + H2O 100% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + myristic acid
-
 ?
3.1.1.13 p-nitrophenyl octanoate + H2O 79% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + octanoic acid
-
 ?
3.1.1.13 p-nitrophenyl palmitate + H2O 68% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + palmitic acid
-
 ?
3.1.1.13 p-nitrophenyl propionate + H2O 17% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + propionic acid
-
 ?
3.1.1.13 p-nitrophenyl stearate + H2O 38% of the activity with cholesteryl linoleate Burkholderia cepacia p-nitrophenol + stearic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.13 ? x * 37000, SDS-PAGE Burkholderia cepacia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.13 45
-
-
 Burkholderia cepacia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.13 4 65
-
 Burkholderia cepacia
3.1.1.13 75
-
 almost complete loss of activity Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.13 7
-
-
 Burkholderia cepacia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.13 5 7.5
-
 Burkholderia cepacia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.13 5.5 12
-
 Burkholderia cepacia