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Literature summary extracted from

  • Castro-Ochoa, L.D.; Rodriguez-Gomez, C.; Valerio-Alfaro, G.; Oliart Ros, R.
    Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11 (2005), Enzyme Microb. Technol., 37, 648-654.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 butanol 70%, 30°C, complete immediate inactivation Geobacillus thermoleovorans
3.1.1.3 Hg+
-
 Geobacillus thermoleovorans
3.1.1.3 K+
-
 Geobacillus thermoleovorans
3.1.1.3 Li+
-
 Geobacillus thermoleovorans
3.1.1.3 Mg2+
-
 Geobacillus thermoleovorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ activates Geobacillus thermoleovorans
3.1.1.3 additional information no effect by Ba2+ and Na+ Geobacillus thermoleovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.3 triolein + H2O Geobacillus thermoleovorans olive oil diolein + oleate
-
 ?
3.1.1.3 triolein + H2O Geobacillus thermoleovorans CCR11 olive oil diolein + oleate
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 2-propanol 70%, 30°C, completely stable for 1 h, loss of 18% activity after 2h Geobacillus thermoleovorans
3.1.1.3 Acetone 70%, 30°C, completely stable for 1 h, loss of 60% activity after 2h Geobacillus thermoleovorans
3.1.1.3 Butanol 70%, 30°C, complete immediate inactivation Geobacillus thermoleovorans
3.1.1.3 Ethanol 70%, 30°C, completely stable for 1 h, loss of 30% activity after 2h Geobacillus thermoleovorans
3.1.1.3 Methanol 70%, 30°C, loss of 35% activity after 2h Geobacillus thermoleovorans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus thermoleovorans
-
 isolated from a hot spring in Mexico
-
3.1.1.3 Geobacillus thermoleovorans CCR11
-
 isolated from a hot spring in Mexico
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native enzyme from cell-free supernatant by isoelectric focusing, PAGE, and ultrafiltration Geobacillus thermoleovorans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 additional information optimal growth at pH 6.5, 55°C in presence of 1% gum arabic, 2.5% olive oil and 0.1% w/v CaCl2 Geobacillus thermoleovorans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Geobacillus thermoleovorans 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Geobacillus thermoleovorans CCR11 4-nitrophenol + laurate
-
 ?
3.1.1.3 triolein + H2O olive oil Geobacillus thermoleovorans diolein + oleate
-
 ?
3.1.1.3 triolein + H2O olive oil Geobacillus thermoleovorans CCR11 diolein + oleate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Geobacillus thermoleovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 60
-
-
 Geobacillus thermoleovorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 30
-
 pH 6.5, 1 h, completely stable, 26 h, over 80% remaining activity Geobacillus thermoleovorans
3.1.1.3 50 60 pH 6.5, 1 h, 75% remaining activity Geobacillus thermoleovorans
3.1.1.3 70
-
 pH 6.5, 1 h, nearly complete loss of activity Geobacillus thermoleovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9 10
-
 Geobacillus thermoleovorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 5 11 purified enzyme, stable Geobacillus thermoleovorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Geobacillus thermoleovorans isoelectric focusing
-
 6