EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.3 | 4-chloromercuribenzoate | complete inhibition at 10 mM, 72% inhibition at 1 mM | Camelus dromedarius | |
3.2.1.3 | Co2+ | 26% inhibition at 1 mM | Camelus dromedarius | |
3.2.1.3 | Cu2+ | 85% inhibition at 1 mM | Camelus dromedarius | |
3.2.1.3 | DTNB | 42% inhibition at 10 mM, no inhibition at 1 mM | Camelus dromedarius | |
3.2.1.3 | Hg2+ | 89% inhibition at 1 mM | Camelus dromedarius | |
3.2.1.3 | iodoacetamide | 78% inhibition at 10 mM, 40% inhibition at 1 mM | Camelus dromedarius | |
3.2.1.3 | additional information | no inhibition by PMSF | Camelus dromedarius | |
3.2.1.3 | Zn2+ | 78% inhibition at 1 mM | Camelus dromedarius |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.3 | 10 | - |
starch | pH 5.5, 37°C | Camelus dromedarius |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.3 | extracellular | the enzyme is secreted by the cells in pancreas to small intestine | Camelus dromedarius | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.3 | Ba2+ | 65% activation at 1 mM | Camelus dromedarius | |
3.2.1.3 | Ca2+ | 74% activation at 1 mM | Camelus dromedarius | |
3.2.1.3 | Mn2+ | 60% activation at 1 mM | Camelus dromedarius | |
3.2.1.3 | Ni2+ | 11% activation at 1 mM | Camelus dromedarius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 58000 | - |
gel filtration | Camelus dromedarius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.3 | Camelus dromedarius | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.3 | native enzyme 93fold from pancreas to homogeneity by glycogen precipitation, ion exchange chromatography, and gel filtration | Camelus dromedarius |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.3 | pancreas | - |
Camelus dromedarius | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 1.02 | - |
pancreas | Camelus dromedarius |
3.2.1.3 | 16.25 | - |
purified enzyme | Camelus dromedarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.3 | alpha-cyclodextrin + H2O | - |
Camelus dromedarius | alpha-cyclodextrin + beta-D-glucose | - |
? | |
3.2.1.3 | amylopectin + H2O | - |
Camelus dromedarius | amylopectin + beta-D-glucose | - |
? | |
3.2.1.3 | dextrin + H2O | - |
Camelus dromedarius | dextrin + beta-D-glucose | - |
? | |
3.2.1.3 | glycogen + H2O | - |
Camelus dromedarius | glycogen + beta-D-glucose | - |
? | |
3.2.1.3 | additional information | substrate specificity overview | Camelus dromedarius | ? | - |
? | |
3.2.1.3 | pectin + H2O | - |
Camelus dromedarius | pectin + beta-D-glucose | - |
? | |
3.2.1.3 | starch + H2O | soluble potato starch | Camelus dromedarius | starch + beta-D-glucose | - |
? | |
3.2.1.3 | xylan + H2O | - |
Camelus dromedarius | xylan + beta-D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.3 | monomer | 1 * 58000, SDS-PAGE | Camelus dromedarius |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.3 | glucoamylase | - |
Camelus dromedarius |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 50 | - |
- |
Camelus dromedarius |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 30 | - |
stable up to | Camelus dromedarius |
3.2.1.3 | 60 | - |
inactivation | Camelus dromedarius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.3 | 5.5 | - |
- |
Camelus dromedarius |