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Literature summary extracted from

  • Athanasopoulos, V.I.; Niranjan, K.; Rastall, R.A.
    The production, purification and characterization of two novel alpha-D-mannosidases from Aspergillus phoenicis (2005), Carbohydr. Res., 340, 609-617.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.163 synthesis the organism is employed in regioselective synthesis of manno-oligosaccharides involving the enzyme Aspergillus phoenicis

General Stability

EC Number General Stability Organism
3.2.1.163 freeze and thaw cycles and freeze drying have a detrimental effect on the purified enzyme with loss of 60% activity Aspergillus phoenicis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.114 EDTA 10 mM Aspergillus phoenicis
3.2.1.114 Mn2+
-
 Aspergillus phoenicis
3.2.1.163 Mg2+
-
 Aspergillus phoenicis
3.2.1.163 Mn2+ strong inhibition Aspergillus phoenicis
3.2.1.163 additional information no inhibition by EDTA at 10 mM Aspergillus phoenicis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.114 14
-
 alpha-D-Man-(1,6)-D-Man
-
 Aspergillus phoenicis
3.2.1.163 14
-
 alpha-D-Manp-(1-6)-D-Manp pH 4.0, 60°C Aspergillus phoenicis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.114 Ca2+ 1 mM Aspergillus phoenicis
3.2.1.114 additional information does not need Ca2+ or any other metal cofactor of those tested Aspergillus phoenicis
3.2.1.163 additional information Ca2+ is not required for activity Aspergillus phoenicis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.114 74000
-
 SDS-PAGE Aspergillus phoenicis
3.2.1.114 81000
-
 native-PAGE Aspergillus phoenicis
3.2.1.114 97000
-
 SDS-PAGE Aspergillus phoenicis
3.2.1.114 110000
-
 native-PAGE Aspergillus phoenicis
3.2.1.163 74000
-
 1 * 74000, SDS-PAGE Aspergillus phoenicis
3.2.1.163 81000
-
 gel filtration Aspergillus phoenicis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.114 Aspergillus phoenicis
-
-
-
3.2.1.163 Aspergillus phoenicis
-
 ATCC 14332, syn. Aspergillus saitoi
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.114 anion-exchange chromatography, chromatofocusing chromatography, gel filtration Aspergillus phoenicis
3.2.1.163 native enzyme 370fold by anion-exchange chromatography, chromatofocussing, and gel filtration Aspergillus phoenicis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.163 48
-
 purified enzyme Aspergillus phoenicis

Storage Stability

EC Number Storage Stability Organism
3.2.1.163 23°C, room temperature, purified enzyme, over 95% remaining activity after 7 days Aspergillus phoenicis
3.2.1.163 4°C, purified enzyme, over 95% remaining activity after 3 months Aspergillus phoenicis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.114 alpha-D-Man-(1,6)-D-Man + H2O
-
 Aspergillus phoenicis alpha-D-mannopyranose
-
 ?
3.2.1.114 alpha-D-Manp-(1,3)-D-Manp + H2O
-
 Aspergillus phoenicis alpha-D-mannopyranose
-
 ?
3.2.1.114 alpha-D-Manp-(1,6)-D-Manp + H2O
-
 Aspergillus phoenicis alpha-D-mannopyranose
-
 ?
3.2.1.114 baker's yeast mannan + H2O
-
 Aspergillus phoenicis ?
-
 ?
3.2.1.114 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Aspergillus phoenicis p-nitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.163 alpha-D-Manp-(1,6)-D-Manp + H2O the enzyme cleaves specifically (1-6)-linked mannobiose and has no activity towards any other linkages Aspergillus phoenicis alpha-D-mannose
-
 ?
3.2.1.163 additional information no activity with p-nitrophenyl-alpha-D-mannopyranoside or baker's yeast mannan Aspergillus phoenicis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.163 monomer 1 * 74000, SDS-PAGE Aspergillus phoenicis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.114 1,3(1,6)-alpha-D-mannosidase
-
 Aspergillus phoenicis
3.2.1.114 1,6-alpha-D-mannosidase
-
 Aspergillus phoenicis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.114 60
-
-
 Aspergillus phoenicis
3.2.1.163 60
-
-
 Aspergillus phoenicis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.163 30
-
 purified enzyme, stable for 24 h, rapid inactivation above Aspergillus phoenicis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.163 4 4.5
-
 Aspergillus phoenicis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.114 4 4.5
-
 Aspergillus phoenicis
3.2.1.163 3 5 36% of maximal activity at pH 3.0, rapid inactivation above pH 5.0 Aspergillus phoenicis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.114 Aspergillus phoenicis for the first isoform of the enzyme, isoelectric focusing
-
 4.3
3.2.1.114 Aspergillus phoenicis method not mentioned
-
 4.6
3.2.1.114 Aspergillus phoenicis for the second isoform of the enzyme, isoelectric focusing
-
 4.8
3.2.1.163 Aspergillus phoenicis isoelectric focusing
-
 4.6