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Literature summary extracted from

  • Shibasaki, T.; Mori, H.; Ozaki, A.
    Cloning of an isozyme of proline 3-hydroxylase and its purification from recombinant Escherichia coli (2000), Biotechnol. Lett., 22, 1967-1973.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.28 expression in Escherichia coli Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.28 Co2+ more than 50% inhibition at 1 mM Streptomyces sp.
1.14.11.28 Cu2+ more than 50% inhibition at 1 mM Streptomyces sp.
1.14.11.28 EDTA more than 50% inhibition at 1 mM Streptomyces sp.
1.14.11.28 Ni2+ more than 50% inhibition at 1 mM Streptomyces sp.
1.14.11.28 Zn2+ more than 50% inhibition at 1 mM Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.28 0.047
-
 2-oxoglutarate
-
 Streptomyces sp.
1.14.11.28 0.083
-
 2-oxoglutarate
-
 Streptomyces sp.
1.14.11.28 0.2
-
 L-proline
-
 Streptomyces sp.
1.14.11.28 0.43
-
 L-proline
-
 Streptomyces sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.28 Fe2+ strictly required Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.28 33150
-
 calculated Streptomyces sp.
1.14.11.28 33570
-
 calculated Streptomyces sp.
1.14.11.28 35000
-
 apparent molecular weight on SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.28 Streptomyces sp.
-
 strainTH1
-
1.14.11.28 Streptomyces sp. TH1
-
 strainTH1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.28 2 chromatographic steps to homogeneity Streptomyces sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.28 cell culture
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.28 7.2
-
-
 Streptomyces sp.
1.14.11.28 12.7
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.28 3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3,4-epoxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 3,4-dehydro-L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3,4-epoxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
 ?
1.14.11.28 L-2-azetidinecarboxylic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxyazetidine-L-2-carboxylic acid + succinate + CO2
-
 ?
1.14.11.28 L-pipecolic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
 ?
1.14.11.28 L-pipecolic acid + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-pipecolic acid + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. cis-3-hydroxy-L-proline + succinate + CO2
-
 ?
1.14.11.28 L-proline + 2-oxoglutarate + O2
-
 Streptomyces sp. TH1 cis-3-hydroxy-L-proline + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.28 proline 3-hydroxylase type I
-
 Streptomyces sp.
1.14.11.28 proline 3-hydroxylase type II
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.28 35
-
-
 Streptomyces sp.
1.14.11.28 40
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.11.28 30
-
 below 30°C activity remained above 85% after 30 min at pH 6.0 Streptomyces sp.
1.14.11.28 40
-
 below 40°C activity remained above 85% after 30 min at pH 6.0 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.28 6
-
-
 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.11.28 5.5 9 activity remained above 85% after 16 h at 4°C Streptomyces sp.
1.14.11.28 5.5 8.5 activity remained above 85% after 16 h at 4°C Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.28 2-oxoglutarate strictly required Streptomyces sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.11.28 Streptomyces sp.
-
-
 4.3
1.14.11.28 Streptomyces sp.
-
-
 4.6