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Literature summary extracted from

  • Aronson, N.N.Jr.; Halloran, B.A
    Alexeyev, M.F.; Zhou, X.E.; Wang, Y.; Meehan, E.J.; Chen, L.: Mutation of a conserved tryptophan in the chitin-binding cleft of Serratia marcescens chitinase A enhances transglycosylation (2006), Biosci. Biotechnol. Biochem., 70, 243-251.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 W167A site-directed mutagenesis, mutation of the conserved tryptophan in the chitin-binding cleft of chitinase A enhances transglycosylation Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 chitin + H2O Serratia marcescens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Serratia marcescens P07254
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.14 chitopentaose + H2O = chitobiose + chitotriose reaction mechanism, Glu315 is the acid/base during hydrolysis, the side-chain of the preceding Asp313 helps to position the N-acetyl moiety of the glycosyl sugar undergoing hydrolysis correctly, mutation of the conserved Tryp167 in the chitin-binding cleft of chitinase A enhances transglycosylation, transglycosylation reaction scheme, overview Serratia marcescens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl beta-N,N',N'',N'''-tetraacetylchitotetraoside + H2O
-
 Serratia marcescens 4-nitrophenol + beta-N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Serratia marcescens 4-nitrophenol + beta-N,N',N''-triacetylchitotriose
-
 ?
3.2.1.14 4-nitrophenyl beta-N,N'-diacetylchitobioside + H2O
-
 Serratia marcescens 4-nitrophenol + beta-N,N'-diacetylchitobiose
-
 ?
3.2.1.14 chitin + H2O
-
 Serratia marcescens ?
-
 ?
3.2.1.14 chito-oligosaccharides + H2O
-
 Serratia marcescens ?
-
 ?
3.2.1.14 additional information wild-type ChiA and mutant ChiAW167A substrate specificity, product determinations, overview, the enzyme also performs transglycosylation with chito-oligosaccharides as acceptors, overview Serratia marcescens ?
-
 ?
3.2.1.14 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Serratia marcescens ?
-
 ?
3.2.1.14 N,N',N''-triacetylchitotriose + H2O
-
 Serratia marcescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA
-
 Serratia marcescens
3.2.1.14 chitinase A
-
 Serratia marcescens