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Literature summary extracted from

  • Arima, K.; Uchikoba, T.; Shimada, M.; Yonezawa, H.; Kaneda, M.
    Purification and some properties of an aminopeptidase from the seeds of Cannabis sativa (2000), Biosci. Biotechnol. Biochem., 64, 1055-1057.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 4-chloromercuriphenylsulfonic acid strong inhibition Cannabis sativa
3.4.11.1 Co2+ slight inhibition Cannabis sativa
3.4.11.1 iodoacetic acid slight inhibition Cannabis sativa
3.4.11.1 Mn2+ slight inhibition Cannabis sativa
3.4.11.1 additional information no inhibition by bestatin, EDTA, EGTA, PMSF, and DFP Cannabis sativa
3.4.11.1 Zn2+ strong inhibition Cannabis sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.194
-
 Pro-4-nitroanilide pH 7.0, 37°C Cannabis sativa
3.4.11.1 0.248
-
 Leu-4-nitroanilide pH 7.0, 37°C Cannabis sativa
3.4.11.1 8.78
-
 Ala-4-nitroanilide pH 7.0, 37°C Cannabis sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 78000
-
 x * 78000, SDS-PAGE Cannabis sativa

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Cannabis sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 native enzyme 474fold from seeds by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, adsorption chromatography, and two steps of leucine affinity chromatography to homogeneity Cannabis sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 seed
-
 Cannabis sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.1 0.18
-
 purified enzyme Cannabis sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Ala-4-nitroanilide + H2O 42% of the activity with Leu-4-nitroanilide Cannabis sativa Ala + 4-nitroaniline
-
 ?
3.4.11.1 Leu-4-nitroanilide + H2O best substrate Cannabis sativa Leu + 4-nitroaniline
-
 ?
3.4.11.1 Leu-Gly-Tyr-Leu + H2O synthetic peptide substrate, successive release of terminal amino acids with descending activity Cannabis sativa Leu + Gly + Tyr + Leu
-
 ?
3.4.11.1 additional information the enzyme acts as aminopeptidase and endopeptidase and might be a cysteine type peptidase, no activity with N-blocked substrate, Gly-4-nitroanilide, Arg-4-nitroanilide, and Lys-4-nitroanilide are poor substrates, Glu-4-nitroanilide is no substrate Cannabis sativa ?
-
 ?
3.4.11.1 Pro-4-nitroanilide + H2O 30% of the activity with Leu-4-nitroanilide Cannabis sativa Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 78000, SDS-PAGE Cannabis sativa

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 HSA
-
 Cannabis sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Cannabis sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 50
-
 60 min, purified enzyme, fairly stable up to Cannabis sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 7
-
 about Cannabis sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.1 6 8 at least 80% of maximal activity at pH 6.0 and pH 8.0 Cannabis sativa